| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4007296 | B | Inhibition of eIF4G-induced full length recombinant human N-terminal His6/SUMO-tagged eIF4A1 RNA dependent ATPase activity expressed in Escherichia coli BL21(DE3) using single stranded poly(U) RNA as substrate after 40 mins by ADP-Glo luminescence assay | Homo sapiens | 4 | ALA4004889 | assay format | Scientific Literature | |
| 2. | ALA4007297 | B | Inhibition of MLN51-induced full length recombinant human N-terminal His6/SUMO-tagged eIF4A3 RNA dependent ATPase activity expressed in Escherichia coli BL21(DE3) using 60 ug/ml single stranded poly(U) RNA as substrate after 30 mins by ADP-Glo luminescence assay | Homo sapiens | 18 | ALA4004889 | assay format | Scientific Literature | |
| 3. | ALA4007298 | B | Inhibition of eIF4G-induced eIF4A2 (unknown origin) RNA dependent ATPase activity expressed in Escherichia coli BL21(DE3) using single stranded poly(U) RNA as substrate after 40 mins by ADP-Glo luminescence assay | Homo sapiens | 4 | ALA4004889 | assay format | Scientific Literature | |
| 4. | ALA4007299 | B | Inhibition of full length recombinant human N-terminal FLAG-His-tagged DHX29 RNA dependent ATPase activity expressed in baculovirus infected Sf9 insect cells using single stranded poly(U) RNA as substrate after 30 mins by ADP-Glo luminescence assay | Homo sapiens | 4 | ALA4004889 | cell-based format | Scientific Literature | |
| 5. | ALA4007300 | B | Inhibition of full length recombinant human N-terminal His-tagged BRR2 RNA dependent ATPase activity expressed in baculovirus infected Sf9 insect cells using single stranded poly(U) RNA as substrate after 30 mins by ADP-Glo luminescence assay | Homo sapiens | 4 | ALA4004889 | cell-based format | Scientific Literature | |
| 6. | ALA4007301 | B | ATP competitive inhibition of MLN51-induced full length recombinant human N-terminal His6/SUMO-tagged eIF4A3 expressed in Escherichia coli BL21(DE3) using varying ATP levels after 30 mins by ADP-Glo luminescence assay | Homo sapiens | 1 | ALA4004889 | assay format | Scientific Literature | |
| 7. | ALA4007302 | B | Inhibition of MLN51-induced full length recombinant human N-terminal His6/SUMO-tagged eIF4A3 expressed in Escherichia coli BL21(DE3) using 1.5 ug/ml single stranded poly(U) RNA after 45 mins by ADP-Glo luminescence assay | Homo sapiens | 1 | ALA4004889 | assay format | Scientific Literature | |
| 8. | ALA4007303 | B | Competitive inhibition of MLN51-induced full length recombinant human N-terminal His6/SUMO-tagged eIF4A3 expressed in Escherichia coli BL21(DE3) using varying levels of single stranded poly(U) RNA as substrate after 105 mins by ADP-Glo luminescence assay | Homo sapiens | 1 | ALA4004889 | assay format | Scientific Literature | |
| 9. | ALA4007304 | B | Binding affinity to full length recombinant human N-terminal His6/SUMO-tagged eIF4A3 expressed in Escherichia coli BL21(DE3) by isothermal titration calorimetric method | Homo sapiens | 1 | ALA4004889 | assay format | Scientific Literature | |
| 10. | ALA4007305 | B | Binding affinity to full length recombinant human N-terminal His6/SUMO-tagged eIF4A3 expressed in Escherichia coli BL21(DE3) administered for 60 to 120 secs by surface plasmon resonance biosensing assay | Homo sapiens | 3 | ALA4004889 | assay format | Scientific Literature | |
| 11. | ALA4007306 | B | Binding affinity to full length recombinant human N-terminal His6/SUMO-tagged eIF4A3 expressed in Escherichia coli BL21(DE3) assessed as change in enthalpy by isothermal titration calorimetric method | Homo sapiens | 1 | ALA4004889 | assay format | Scientific Literature | |
| 12. | ALA4007307 | B | Binding affinity to full length recombinant human N-terminal His6/SUMO-tagged eIF4A3 expressed in Escherichia coli BL21(DE3) assessed as association rate constant administered for 60 to 120 secs by surface plasmon resonance biosensing assay | Homo sapiens | 3 | ALA4004889 | assay format | Scientific Literature | |
| 13. | ALA4007308 | B | Binding affinity to full length recombinant human N-terminal His6/SUMO-tagged eIF4A3 expressed in Escherichia coli BL21(DE3) assessed as dissociation rate constant administered for 60 to 120 secs by surface plasmon resonance biosensing assay | Homo sapiens | 3 | ALA4004889 | assay format | Scientific Literature | |
| 14. | ALA4007309 | B | Binding affinity to full length recombinant human N-terminal His6/SUMO-tagged eIF4A3 expressed in Escherichia coli BL21(DE3) assessed as dissociation half-life administered for 60 to 120 secs by surface plasmon resonance biosensing assay | Homo sapiens | 3 | ALA4004889 | assay format | Scientific Literature | |
| 15. | ALA4007310 | B | Binding affinity to full length recombinant human N-terminal His6/SUMO-tagged eIF4A3 expressed in Escherichia coli BL21(DE3) assessed as change in Gibbs free energy by isothermal titration calorimetric method | Homo sapiens | 1 | ALA4004889 | assay format | Scientific Literature | |
| 16. | ALA4007311 | B | Binding affinity to full length recombinant human N-terminal His6/SUMO-tagged eIF4A3 expressed in Escherichia coli BL21(DE3) assessed as change in entropy by isothermal titration calorimetric method | Homo sapiens | 1 | ALA4004889 | assay format | Scientific Literature | |
| 17. | ALA4007312 | B | Inhibition of full length recombinant human N-terminal His6/SUMO-tagged eIF4A3 helicase activity expressed in Escherichia coli BL21(DE3) assessed as reduction in RNA unwinding up to 300 uM using (5'-(FITC)-GCUUUACGGUGC-3')/(5'-GGGGAGAAAAACAAAACAAAACUAGCACCGUAAAGCACGC-3') RNA as substrate in presence of ATP/MLN51 after 30 mins by gel electrophoresis method | Homo sapiens | 2 | ALA4004889 | assay format | Scientific Literature |
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