#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4037929	B	Inhibition of anti-hMD2 antibody binding to C-terminal His6-tagged human MD2 expressed in Pichia pastoris GS115 at 20 uM preincubated for 2 hrs followed by antibody addition by sandwich ELISA	Homo sapiens	4		assay format	Scientific Literature
2.	ALA4037930	B	Displacement of biotinylated LPS from C-terminal His6-tagged human MD2 expressed in Pichia pastoris GS115 at 160 uM after 1.5 hrs by ELISA	Homo sapiens	4		assay format	Scientific Literature
3.	ALA4037931	B	Displacement of bis-ANS from C-terminal His6-tagged human MD2 expressed in Pichia pastoris GS115 by fluorescence spectroscopic assay	Homo sapiens	3		assay format	Scientific Literature
4.	ALA4037932	B	Binding to C-terminal His6-tagged human MD2 expressed in Pichia pastoris GS115 by surface plasmon resonance assay	Homo sapiens	4		assay format	Scientific Literature
5.	ALA4037933	B	Antagonist activity at human TLR4 expressed in HEK blue cells assessed as inhibition of LPS-induced NF-kappaB activation-mediated SEAP production preincubated for 30 mins followed by LPS addition measured after overnight incubation in absence of light by NF-kappaB reporter gene assay	Homo sapiens	3		cell-based format	Scientific Literature
6.	ALA4037934	B	Antagonist activity at human TLR4 expressed in mouse RAW blue cells assessed as inhibition of LPS-induced NF-kappaB activation-mediated SEAP production preincubated for 30 mins followed by LPS addition measured after 16 hrs by NF-kappaB reporter gene assay	Homo sapiens	3		cell-based format	Scientific Literature
7.	ALA4037935	A	Cytotoxicity against HEK-Blue cells assessed as reduction in cell viability at 10 uM after overnight incubation by MTT assay	Homo sapiens	3		cell-based format	Scientific Literature
8.	ALA4037936	A	Cytotoxicity against human THP1 cells assessed as reduction in cell viability up to 10 uM after 24 hrs in presence of LPS by MTT asssay	Homo sapiens	4		cell-based format	Scientific Literature
9.	ALA4037937	B	Inhibition of TLR4/MD2 interaction in human THP1 cells assessed as reduction of LPS-induced IL8 production at 10 uM preincubated for 1 hr followed by LPS stimulation measured after 16 hrs by ELISA	Homo sapiens	4		cell-based format	Scientific Literature
10.	ALA4037938	B	Inhibition of TLR4/MD2 interaction in human THP1 cells assessed as reduction of LPS-induced p65 NF-kappaB phosphorylation up to 10 uM preincubated for 1 hr followed by LPS stimulation measured after 16 hrs by immunoblot method	Homo sapiens	1		cell-based format	Scientific Literature
11.	ALA4037939	B	Inhibition of TLR4/MD2 interaction in human THP1 cells assessed as reduction of LPS-induced IL6 production up to 10 uM preincubated for 1 hr followed by LPS stimulation measured after 16 hrs by ELISA	Homo sapiens	1		cell-based format	Scientific Literature
12.	ALA4037940	B	Inhibition of TLR4/MD2 interaction in human THP1 cells assessed as reduction of LPS-induced IL1beta production up to 10 uM preincubated for 1 hr followed by LPS stimulation measured after 16 hrs by ELISA	Homo sapiens	1		cell-based format	Scientific Literature
13.	ALA4037941	B	Inhibition of anti-hMD2 antibody binding to C-terminal His6-tagged human MD2 expressed in Pichia pastoris GS115 preincubated for 2 hrs followed by antibody addition by sandwich ELISA	Homo sapiens	2		assay format	Scientific Literature
14.	ALA4037942	B	Displacement of biotinylated LPS from C-terminal His6-tagged human MD2 expressed in Pichia pastoris GS115 after 1.5 hrs by ELISA	Homo sapiens	2		assay format	Scientific Literature
15.	ALA4037944	A	Agonist activity at human TLR4 expressed in mouse RAW blue cells assessed as increase in NF-kappaB activation-mediated SEAP production up to 10 uM measured after 20 hrs by SEAP reporter gene assay	Homo sapiens	4		cell-based format	Scientific Literature
16.	ALA4037946	A	Cytotoxicity against human THP1 cells assessed as reduction in cell viability up to 10 uM after 24 hrs in absence of LPS by MTT asssay	Homo sapiens	4		cell-based format	Scientific Literature