Inhibition of Influenza A virus A/Udorn/72 wild-type M2 proton channel expressed in Xenopus laevis oocytes at 100 uM at pH 5.5 after 2 mins at -20 mV holding potential by two-electrode voltage clamp assay relative to control
Inhibition of Influenza A virus A/Udorn/72 wild-type M2 proton channel expressed in Xenopus laevis oocytes at pH 5.5 after 2 mins at -20 mV holding potential by two-electrode voltage clamp assay
Inhibition of Influenza A virus A/Udorn/72 amantadine-resistant M2 proton channel V27A mutant expressed in Xenopus laevis oocytes at 100 uM at pH 5.5 after 2 mins at -20 mV holding potential by two-electrode voltage clamp assay relative to control
Inhibition of Influenza A virus A/Udorn/72 amantadine-resistant M2 proton channel V27A mutant expressed in Xenopus laevis oocytes at pH 5.5 after 2 mins at -20 mV holding potential by two-electrode voltage clamp assay relative to control
Inhibition of Influenza A virus A/Udorn/72 amantadine-resistant M2 proton channel S31N mutant expressed in Xenopus laevis oocytes at 100 uM at pH 5.5 after 2 mins at -20 mV holding potential by two-electrode voltage clamp assay relative to control
Antiviral activity against Influenza A virus A//HK/7/87 (H3N2) expressing wild-type M2 proton channel infected in MDCK cells assessed as reduction in plaque formation preincubated with virus for 1 hr followed by addition to cells measured after 72 hrs by crystal violet staining based assay
Antiviral activity against Influenza A virus A//HK/7/87 (H3N2) expressing wild-type M2 proton channel infected in MDCK cells assessed as reduction in viral yield after 24 hrs by RT-qPCR method
Antiviral activity against Influenza A virus A/WSN/33 (H1N1) expressing M2 proton channel N31S/V27A double mutant infected in MDCK cells assessed as reduction in plaque formation measured at 46 hrs by crystal violet staining based assay
Inhibition of Influenza A virus A/Udorn/307/72 H3N2 wild-type M2 proton channel expressed in Xenopus laevis oocytes assessed as association rate constant at pH 5.5 after 2 mins at -20 mV holding potential by two-electrode voltage clamp assay
Inhibition of Influenza A virus A/Udorn/307/72 H3N2 wild-type M2 proton channel expressed in Xenopus laevis oocytes assessed as dissociation rate constant at pH 5.5 incubated for 2 mins followed by compound washout at -20 mV holding potential by two-electrode voltage clamp assay
Inhibition of Influenza A virus A/Udorn/307/72 H3N2 amantadine-resistant M2 proton channel V27A mutant expressed in Xenopus laevis oocytes assessed as association rate constant at pH 5.5 after 2 mins at -20 mV holding potential by two-electrode voltage clamp assay
Inhibition of Influenza A virus A/Udorn307/72 H3N2 amantadine-resistant M2 proton channel V27A mutant expressed in Xenopus laevis oocytes assessed as dissociation rate constant at pH 5.5 incubated for 2 mins followed by compound washout at -20 mV holding potential by two-electrode voltage clamp assay
Inhibition of Influenza A virus A/Udorn/307/72 H3N2 wild-type M2 proton channel expressed in Xenopus laevis oocytes assessed as dissociation time at pH 5.5 incubated for 2 mins followed by compound washout at -20 mV holding potential by two-electrode voltage clamp assay
Potency index, ratio of compound K to amantadine K for Influenza A virus A/Udorn/307/72 H3N2 wild-type M2 proton channel expressed in Xenopus laevis oocytes assessed as association rate constant
Potency index, ratio of amantadine K to compound K for Influenza A virus A/Udorn/307/72 H3N2 wild-type M2 proton channel expressed in Xenopus laevis oocytes assessed as dissociation rate constant