#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4015867	Binding	Inhibition of human TMEM16A expressed in FRT cells co-expressing iodide sensitive fluorescent protein YFP-H148Q/I152L/F46L assessed as reduction in ATP-induced Ca2+ activation-mediated chloride current by measuring decrease in iodide influx preincubated for 10 mins followed by ATP/iodide addition by fluorescence assay	Homo sapiens	50	ALA4014325	cell-based format	Scientific Literature
2.	ALA4015868	Binding	Inhibition of human TMEM16A expressed in FRT cells assessed as reduction of ATP-induced in chloride conductance preincubated for 5 mins followed by ATP addition by short-circuit current assay	Homo sapiens	9	ALA4014325	cell-based format	Scientific Literature
3.	ALA4015869	Binding	Inhibition of human TMEM16B expressed in FRT cells co-expressing iodide sensitive fluorescent protein YFP-H148Q/I152L/F46L assessed as reduction in ATP-induced Ca2+ activation-mediated chloride current by measuring decrease in iodide influx preincubated for 10 mins followed by ATP/iodide addition by fluorescence assay	Homo sapiens	8	ALA4014325	cell-based format	Scientific Literature
4.	ALA4015871	ADME	Cytotoxicity against human HT-29 cells assessed as cell survival at 5 uM after 8 hrs by alamar blue assay relative to control	Homo sapiens	9	ALA4014325	cell-based format	Scientific Literature
5.	ALA4015872	Binding	Inhibition of human wild type CFTR expressed in FRT cells co-expressing halide sensitive YFP-H148Q assessed as reduction in forskolin induced cAMP activated chloride conductance by measuring decrease in iodide influx after 15 mins by fluorescence assay	Homo sapiens	9	ALA4014325	cell-based format	Scientific Literature
6.	ALA4015874	Binding	Inhibition of calcium activated chloride channel in ATP-activated human HT-29 cells expressing YFP-H148Q/152L assessed as reduction in iodide influx preincubated for 10 mins followed by ATP addition by fluorescence assay	Homo sapiens	9	ALA4014325	assay format	Scientific Literature
7.	ALA4015875	Binding	Inhibition of human TMEM16A expressed in FRT cells assessed as reduction in Ca2+ activated chloride current at 250 nM at -60 mV holding potential by inside-out patch-clamp method	Homo sapiens	1	ALA4014325	cell-based format	Scientific Literature
8.	ALA4015876	Binding	Reversible inhibition of human TMEM16A expressed in FRT cells assessed as recovery of Ca2+ activated chloride current at 250 nM preincubated with cells followed by compound washout at -60 mV holding potential by inside-out patch-clamp method	Homo sapiens	1	ALA4014325	cell-based format	Scientific Literature
9.	ALA4015877	Binding	Inhibition of human TMEM16A expressed in FRT cells assessed as reduction in ionomycin activated chloride conductance at 1 uM preincubated for 5 mins followed by ionomycin addition by short-circuit current assay	Homo sapiens	1	ALA4014325	cell-based format	Scientific Literature
10.	ALA4015879	Binding	Inhibition of human TMEM16A expressed in FRT cells assessed as alteration of ATP stimulated cytoplasmic calcium elevation at 1 uM preincubated for 3 mins followed by ATP addition by Fluo-4 fluorescence assay	Homo sapiens	1	ALA4014325	cell-based format	Scientific Literature
11.	ALA4015880	ADME	Metabolic stability in rat liver microsomes assessed as compound remaining in presence of NADPH at 10 uM after 180 mins by LC/MS analysis	Rattus norvegicus	1	ALA4014325	assay format	Scientific Literature
12.	ALA4015881	Physicochemical	Kinetic aqueous solubility of compound in PBS by titration method		1	ALA4014325	small-molecule physicochemical format	Scientific Literature
13.	ALA4015882	Binding	Inhibition of TMEM16A in CD1 mouse ileum assessed as reduction in intestinal smooth muscle contraction	Mus musculus	1	ALA4014325	assay format	Scientific Literature
14.	ALA4015883	Physicochemical	Stability of compound in aqueous solution at 100 uM after 1 day by ESI-LCMS analysis		1	ALA4014325	small-molecule physicochemical format	Scientific Literature
15.	ALA4015884	Physicochemical	Stability of compound in aqueous solution at 100 uM after 2 days by ESI-LCMS analysis		1	ALA4014325	small-molecule physicochemical format	Scientific Literature
16.	ALA4015885	Physicochemical	Drug metabolism in aqueous solution assessed as 2-oxo-2-(3-(o-tolylcarbamoyl)-5,6,7,8-tetrahydro-4H-cyclohepta[b]thiophen-2-ylamino)acetyl fluoride formation after 3 days by ESI-LCMS analysis		1	ALA4014325	small-molecule physicochemical format	Scientific Literature
17.	ALA4015886	Physicochemical	Drug metabolism in aqueous solution assessed as 2-(2,2-difluoro-2-hydroxyacetamido)-N-o-tolyl-5,6,7,8-tetrahydro-4H-cyclohepta[b]thiophene-3-carboxamide formation after 3 days by ESI-LCMS analysis		1	ALA4014325	small-molecule physicochemical format	Scientific Literature
18.	ALA4015889	Binding	Selectivity ratio of IC50 for human TMEM16B expressed in FRT cells co-expressing iodide sensitive fluorescent protein YFP-H148Q/I152L/F46L to IC50 for human TMEM16A expressed in FRT cells co-expressing iodide sensitive fluorescent protein YFP-H148Q/I152L/F46L	Homo sapiens	2	ALA4014325	cell-based format	Scientific Literature
19.	ALA4015890	Binding	Inhibition of human TMEM16A expressed in FRT cells assessed as reduction in carbachol activated chloride conductance at 1 uM preincubated for 5 mins followed by carbachol addition by short-circuit current assay	Homo sapiens	1	ALA4014325	cell-based format	Scientific Literature
20.	ALA4015891	Physicochemical	Stability of compound in DMSO		1	ALA4014325	small-molecule physicochemical format	Scientific Literature