Inhibition of recombinant 6x-His-tagged JNK3 (39 to 402 residues) (unknown origin) expressed in Escherichia coli BL21(DE3) assessed as reduction in ATF2 phosphorylation measured after 50 mins by ELISA
Inhibition of GST-His tagged human p38alpha expressed in Escherichia coli assessed as reduction in ATF2 phosphorylation measured after 60 mins by ELISA
Selectivity ratio of IC50 for GST-His tagged human p38alpha expressed in Escherichia coli to IC50 for recombinant 6x-His-tagged JNK3 (39 to 402 residues) (unknown origin) expressed in Escherichia coli BL21(DE3)
Drug metabolism in human liver microsomes at 100 uM preincubated with NADPH regenerating system for 5 mins followed by addition of liver microsomes measured after 180 mins by LC-MS analysis
Covalent binding affinity to recombinant 6x-His-tagged JNK3 (39 to 402 residues) (unknown origin) expressed in Escherichia coli BL21(DE3) at 5 molar excess after 1 hr by LC-muESI-QTOF mass spectrometry