| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4048125 | B | Inhibition of Keap1-Nrf2 protein-protein interaction in MEF assessed as increase in Nrf2 activation mediated NQO1 mRNA level at 100 uM after 4 hrs by qRT-PCR method | Mus musculus | 1 | assay format | Scientific Literature | ||
| 2. | ALA4048127 | B | Binding affinity to His-tagged Keap1 DC domain (321 to 609 residues) (unknown origin) expressed in Escherichia coli assessed as reduction in protein interaction with FAM-labeled Nrf2 peptide after 30 mins by fluorescence polarization assay | Homo sapiens | 8 | assay format | Scientific Literature | ||
| 3. | ALA4048128 | A | Metabolic stability in human liver microsomes assessed as parent compound remaining at 1 uM preincubated for 3 mins followed by NADP+ addition measured after 30 mins by LC-MS assay | Homo sapiens | 8 | assay format | Scientific Literature | ||
| 4. | ALA4048129 | A | Cytotoxicity against human HepG2 cells assessed as reduction in cell viability at 50 uM after 24 hrs by WST8 assay | Homo sapiens | 8 | cell-based format | Scientific Literature | ||
| 5. | ALA4376060 | B | Binding affinity to recombinant human KEAP1 Kelch domain (321 to 609 residues) expressed in Escherichia coli BL21 (DE3) assessed as thermal stabilization by sypro orange dye based thermal shift assay | Homo sapiens | 19 | assay format | Scientific Literature | ||
| 6. | ALA4376062 | B | Binding affinity to recombinant human KEAP1 Kelch domain (321 to 609 residues) expressed in Escherichia coli BL21 (DE3) by surface plasmon resonance analysis | Homo sapiens | 21 | assay format | Scientific Literature | ||
| 7. | ALA4376064 | B | Binding affinity to recombinant human KEAP1 Kelch domain (321 to 609 residues) expressed in Escherichia coli BL21 (DE3) assessed as association rate constant at 660 nM by surface plasmon resonance analysis | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 8. | ALA4376066 | B | Binding affinity to Keap1 in mouse Hepa1c1c7 cells assessed as reduction in Keap1 interaction by measuring induction of NQO1 activity measured after 24 hrs | Mus musculus | 9 | cell-based format | Scientific Literature | ||
| 9. | ALA4376067 | B | Inhibition of Cys5-LDEETGEFL-NH2 binding to recombinant human KEAP1 Kelch domain (321 to 609 residues) expressed in Escherichia coli BL21 (DE3) measured after 10 to 15 mins by fluorescence polarization assay | Homo sapiens | 25 | assay format | Scientific Literature | ||
| 10. | ALA4376074 | B | Binding affinity to recombinant human KEAP1 Kelch domain (321 to 609 residues) expressed in Escherichia coli BL21 (DE3) assessed as dissociation rate constant at 660 nM by surface plasmon resonance analysis | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 11. | ALA4376091 | U | Redox cycling activity of the compound assessed as H2O2 production measured after 15 mins in presence of TCEP by phenol red reagent based horseradish peroxidase coupled assay | 21 | assay format | Scientific Literature | |||
| 12. | ALA4376093 | B | Inhibition of Cys5-LDEETGEFL-NH2 binding to recombinant human KEAP1 Kelch domain (321 to 609 residues) expressed in Escherichia coli BL21 (DE3) measured after 10 to 15 mins in presence of 0.01% Triton X-100 by fluorescence polarization assay | Homo sapiens | 13 | assay format | Scientific Literature | ||
| 13. | ALA4376101 | B | Binding affinity to recombinant human KEAP1 Kelch domain (321 to 609 residues) expressed in Escherichia coli BL21 (DE3) assessed as change in melting temperature by sypro orange dye based thermal shift assay | Homo sapiens | 11 | assay format | Scientific Literature |
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