# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4020060 | B | Binding affinity to N-terminally 6xHis-Smt3 tagged human Ube2T C86K/K91R/K95R mutant expressed in Escherichia coli by ITC method | Homo sapiens | 2 | ALA4017530 | assay format | Scientific Literature | |
2. | ALA4020061 | B | Binding affinity to N-terminally 6xHis-Smt3 tagged FANCL RING fused human Ube2T C86K/K91R/K95R mutant expressed in Escherichia coli by ITC method | Homo sapiens | 5 | ALA4017530 | assay format | Scientific Literature | |
3. | ALA4020062 | B | Binding affinity to N-terminally 6xHis-Smt3 tagged Ub fused human Ube2T C86K/K91R/K95R mutant expressed in Escherichia coli at 1.5 mM by ITC method | Homo sapiens | 1 | ALA4017530 | assay format | Scientific Literature | |
4. | ALA4020063 | B | Binding affinity to N-terminally 6xHis-Smt3 tagged human Ube2T C86K/K91R/K95R mutant expressed in Escherichia coli in presence of EDTA by ITC method | Homo sapiens | 1 | ALA4017530 | assay format | Scientific Literature | |
5. | ALA4020064 | B | Inhibition of N-terminally 6xHis-Smt3 tagged human Ube2T C86K/K91R/K95R mutant expressed in Escherichia coli charging with ubiquitin after 10 mins in presence of E1 and ATP by coomassie staining based SDS PAGE analysis | Homo sapiens | 1 | ALA4017530 | assay format | Scientific Literature | |
6. | ALA4020065 | B | Inhibition of recombinant human E1 charging with ubiquitin at 20 to 100 uM after 10 mins in presence of ATP by coomassie staining based SDS PAGE analysis | Homo sapiens | 1 | ALA4017530 | single protein format | Scientific Literature | |
7. | ALA4020066 | B | Binding affinity to biotin labeled Ube2T C86K/K91R/K95R mutant (unknown origin) assessed as association and dissociation response at 2 uM by biolayer interferometry analysis | Homo sapiens | 1 | ALA4017530 | assay format | Scientific Literature | |
8. | ALA4020067 | B | Binding affinity to N-terminally 6xHis-Smt3 tagged human Ube2T C86K/K91R/K95R mutant expressed in Escherichia coli by DSF method | Homo sapiens | 6 | ALA4017530 | assay format | Scientific Literature | |
9. | ALA4020068 | B | Binding affinity to human 15N-labelled C-terminal flexible tail lacking Ube2T (1 to 154 residues) (unknown origin) at 500 uM by NMR spectrocopy | Homo sapiens | 1 | ALA4017530 | single protein format | Scientific Literature | |
10. | ALA4020069 | B | Reversible binding affinity to human 15N-labelled C-terminal flexible tail lacking Ube2T (1 to 154 residues) at 100 to 500 uM measured after overnight dialysis by NMR spectrocopy | Homo sapiens | 1 | ALA4017530 | single protein format | Scientific Literature |