In vivo antibacterial activity against Staphylococcus aureus Newman infected in BALB/c mouse model assessed as mouse survival rate at 180 mg/kg dosed every 12 hrs time interval measured on day 3 post infection
Antibacterial activity against Staphylococcus aureus NRS271 infected in BALB/c mouse assessed as reduction of bacterial burden in heart at 0.1 mg, ip bid for 4.5 days administered 6 hrs post bacterial infection measured 108 hrs post infection relative to control
Inhibition of CrtN in Staphylococcus aureus USA300 LAC assessed as H2O2-induced bacterial death by measuring fold reduction in survival rate at 1 uM preincubated for 24 hrs followed by H2O2 addition measured after 30 mins by serial dilution method
Inhibition of Staphylococcus aureus Newman 6His-tagged CrtN expressed in Escherichia coli BL21(DE3) assessed as reduction in staphyloxanthin levels using diapophytoene as substrate after overnight incubation by spectrophotometric analysis
Inhibition of CrtN in Staphylococcus aureus Mu50 assessed as H2O2-induced bacterial death by measuring fold reduction in survival rate at 1 uM preincubated for 24 hrs followed by H2O2 addition measured after 30 mins by serial dilution method
Antibacterial activity against non-pigmented Staphylococcus aureus Newman assessed as H2O2-induced bacterial death by measuring survival rate at 1 uM preincubated for 24 hrs followed by H2O2 addition measured after 30 mins by serial dilution method (Rvb = 33.3%)