# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4003653 | Binding | Displacement of biotinylated ephrin-A1-Fc from recombinant mouse EphA2 receptor preincubated for 1 hr followed by biotinylated ephrin-A1-Fc addition measured after 4 hrs by ELISA method | Mus musculus | 17 | ALA4002595 | single protein format | Scientific Literature | |
2. | ALA4003655 | Physicochemical | n-Octanol-buffer partition co-efficient, log D of the compound at pH 7.4 incubated overnight by shake-flask method | 3 | ALA4002595 | small-molecule physicochemical format | Scientific Literature | ||
3. | ALA4003656 | Physicochemical | Kinetic solubility in MOPS buffer at pH 7.4 measured after 4 hrs by HPLC-ESI-MS/MS analysis | 3 | ALA4002595 | small-molecule physicochemical format | Scientific Literature | ||
4. | ALA4003657 | ADME | Stability in mouse plasma assessed as compound remaining at 1 uM after 24 hrs by HPLC-ESI-MS/MS analysis | Mus musculus | 3 | ALA4002595 | cell-free format | Scientific Literature | |
5. | ALA4003658 | ADME | Half-life in mouse liver microsomes at 1 uM in presence of NADPH by HPLC-ESI-MS/MS analysis | Mus musculus | 3 | ALA4002595 | assay format | Scientific Literature | |
6. | ALA4003659 | ADME | Cmax in mouse at 30 mg/kg, administered as single dose via oral gavage by HPLC-ESI-MS/MS analysis | Mus musculus | 3 | ALA4002595 | organism-based format | Scientific Literature | |
7. | ALA4003660 | ADME | Tmax in mouse at 30 mg/kg, administered as single dose via oral gavage by HPLC-ESI-MS/MS analysis | Mus musculus | 2 | ALA4002595 | organism-based format | Scientific Literature | |
8. | ALA4003662 | ADME | Drug uptake in mouse plasma at 30 mg/kg, administered as single dose via oral gavage after 24 hrs by HPLC-ESI-MS/MS analysis | Mus musculus | 1 | ALA4002595 | organism-based format | Scientific Literature | |
9. | ALA4684873 | Binding | Displacement of biotinylated ephrin-A1-Fc from recombinant mouse c-terminal His6-tagged EphA2 Fc (Ala22 to Ala535 residues) preincubated for 1 hr followed by ephrin-A1-FC addition and measured after 4 hrs by ELISA | Mus musculus | 18 | ALA4680187 | single protein format | Scientific Literature | |
10. | ALA4684874 | Binding | Antagonist activity at EphA2-Fc in human PC3 cells assessed as reduction in ephrin-A1-Fc stimulated EphA2 phosphorylation preincubated for 20 mins followed by ephrin-A1 stimulation and measured after 20 mins by ELISA | Homo sapiens | 5 | ALA4680187 | cell-based format | Scientific Literature | |
11. | ALA4684875 | ADME | Cytotoxicity against human PC3 cells after 2 hrs by LDH assay | Homo sapiens | 5 | ALA4680187 | cell-based format | Scientific Literature | |
12. | ALA4684876 | Binding | Competitive inhibition of biotinylated ephrin-A1-Fc binding to EphA2 (unknown origin) | Homo sapiens | 3 | ALA4680187 | single protein format | Scientific Literature | |
13. | ALA4684893 | Functional | Antiangiogenic activity in Leghorn chicken fertilized egg chorioallantoic membrane assessed as reduction in unstimulated neovascularization at 10 uM measured after 3 days by CAM assay | Gallus gallus | 2 | ALA4680187 | tissue-based format | Scientific Literature |