# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA3999063 | B | Inhibition of 5'-biotin-labeled c-Myc Pu18 G-quadruplex DNA (unknown origin) interaction with His-tagged NM23-H2 assessed as ratio of Kd for NM23-H2-DNA complex in presence of compound to Kd for NM2-H2-DNA complex in absence of compound at 20 uM preincubated with NM23-H2 for 1 hr followed by addition of DNA measured after overnight incubation by ELISA | Not specified | 13 | assay format | Scientific Literature | ||
2. | ALA3999066 | B | Stabilization of 5'-FAM/3'-TAMRA labeled c-Myc Pu18 G-quadruplex DNA (unknown origin) assessed as change in melting temperature at 1 uM after overnight incubation by FRET assay | Not specified | 4 | assay format | Scientific Literature | ||
3. | ALA3999072 | B | Binding affinity to NT647-labeled His-tagged NM23-H2 (unknown origin) expressed in Escherichia coli BL21(DE3) after 30 mins by microscale thermophoresis method | Homo sapiens | 2 | assay format | Scientific Literature | ||
4. | ALA4018538 | B | Binding affinity to NM23-H2 (unknown origin) by SPR analysis | Homo sapiens | 31 | single protein format | Scientific Literature | ||
5. | ALA4018539 | B | Binding affinity to N-terminal NT-647-NHS labeled NM23-H2 (unknown origin) after 30 mins by MST analysis | Homo sapiens | 47 | single protein format | Scientific Literature | ||
6. | ALA4018549 | B | Binding affinity to Htg21 telomeric G-quadruplex DNA (unknown origin) at 40 uM after 30 mins by SPR analysis | Not specified | 47 | assay format | Scientific Literature | ||
7. | ALA4018550 | B | Binding affinity to biotin-labeled 18-mer duplex DNA hairpin (unknown origin) at 40 uM after 30 mins by SPR analysis | Not specified | 47 | assay format | Scientific Literature | ||
8. | ALA4018551 | B | Binding affinity to NM23-H2 (unknown origin) assessed as ratio of Kd for protein interaction with biotin-labeled Pu22 c-myc G-quadruplex DNA in presence and absence of compound after 1 hr by ELISA | Homo sapiens | 17 | single protein format | Scientific Literature | ||
9. | ALA4018617 | B | Binding affinity to NM23-H2 in human SiHa cells assessed as disruption of protein interaction with c-myc G-quadruplex DNA by measuring decrease in c-MYC mRNA level at 2 uM after 9 hrs by RT-PCR analysis | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
10. | ALA4018618 | B | Binding affinity to NM23-H2 in human SiHa cells assessed as disruption of protein interaction with c-myc G-quadruplex DNA by measuring decrease in c-MYC protein expresson at 2 uM after 48 hrs by Western blot analysis | Homo sapiens | 2 | cell-based format | Scientific Literature |