# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4051531 | Binding | Inhibition of His-tagged BRPF1 (626 to 740 residues) (unknown origin) preincubated for 15 mins followed by addition of C-terminal biotinylated synthetic K5,12 diacetylated histone H4 (1 to 20 residues) peptide as substrate measured after 1 hr by TR-FRET assay | Homo sapiens | 27 | ALA4049437 | single protein format | Scientific Literature | |
2. | ALA4051532 | Binding | Inhibition of human BRPF2 (563 to 688 residues) expressed in Escherichia coli BL21 (DE3)-R3-pRARE2 preincubated for 15 mins followed by addition of C-terminal biotinylated synthetic K5,12 diacetylated histone H4 (1 to 20 residues) peptide as substrate measured after 1 hr by TR-FRET assay | Homo sapiens | 27 | ALA4049437 | assay format | Scientific Literature | |
3. | ALA4051536 | Binding | Inhibition of human BRD4 BD1 (67 to 152 residues) preincubated for 15 mins followed by addition of C-terminal biotinylated synthetic K5,8,12,16 tetra-acetylated histone H4 (1 to 20 residues) peptide as substrate measured after 1 hr by TR-FRET assay | Homo sapiens | 27 | ALA4049437 | single protein format | Scientific Literature | |
4. | ALA4051537 | Binding | Inhibition of BRD4 BD2 (unknown origin) preincubated for 15 mins followed by addition of C-terminal biotinylated synthetic K5,8,12,16 tetra-acetylated histone H4 (1 to 20 residues) peptide as substrate measured after 1 hr by TR-FRET assay | Homo sapiens | 27 | ALA4049437 | single protein format | Scientific Literature | |
5. | ALA4051539 | Binding | Inhibition of Halo-tagged histone H4 binding to C-terminally NanoLuc luciferase conjugated human BRPF2 BD (560 to 666 residues) expressed in human HCT116 cells after 4 to 24 hrs by BRET assay | Homo sapiens | 14 | ALA4049437 | cell-based format | Scientific Literature | |
6. | ALA4051542 | Binding | Inhibition of Halo-tagged histone H4 binding to C-terminally NanoLuc luciferase conjugated BRPF1 BD (626 to 732 residues) (unknown origin) expressed in human HCT116 cells after 4 to 24 hrs by BRET assay | Homo sapiens | 5 | ALA4049437 | cell-based format | Scientific Literature |