| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4030854 | B | Inhibition of UbcH5c (unknown origin) expressed in human 293T cells assessed as decrease in TNF-alpha-mediated NF-kB activation at 5 uM preincubated for 2 hrs followed by TNF-alpha stimulation for 8 hrs by luciferase reporter gene assay relative to control | Homo sapiens | 14 | cell-based format | Scientific Literature | ||
| 2. | ALA4030855 | A | Cytotoxicity against human 293T cells assessed as inhibition of cell viability at 5 uM by CCK-8 assay relative to control | Homo sapiens | 14 | cell-based format | Scientific Literature | ||
| 3. | ALA4030856 | A | Clearance in Sprague-Dawley rat at 1 mg/kg, iv by LC-MS/MS analysis | Rattus norvegicus | 1 | organism-based format | Scientific Literature | ||
| 4. | ALA4030857 | A | AUC (0 to infinity) in Sprague-Dawley rat at 1 mg/kg, iv by LC-MS/MS analysis | Rattus norvegicus | 1 | organism-based format | Scientific Literature | ||
| 5. | ALA4030858 | A | Half life in Sprague-Dawley rat at 1 mg/kg, iv by LC-MS/MS analysis | Rattus norvegicus | 1 | organism-based format | Scientific Literature | ||
| 6. | ALA4030859 | A | Volume of distribution at steady state in Sprague-Dawley rat at 1 mg/kg, iv by LC-MS/MS analysis | Rattus norvegicus | 1 | organism-based format | Scientific Literature | ||
| 7. | ALA4030860 | B | Inhibition of patient derived UbcH5c in synovial fibroblast cells assessed as decrease in TNF- alpha induced IL-1 mRNA level preincubated for 2 hrs followed by TNF-alpha addition for 3 hrs by qPCR analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 8. | ALA4030861 | B | Inhibition of patient derived UbcH5c in synovial fibroblast cells assessed as decrease in TNF- alpha induced MCP-1 mRNA level preincubated for 2 hrs followed by TNF-alpha addition for 3 hrs by qPCR analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 9. | ALA4030862 | B | Inhibition of patient derived UbcH5c in synovial fibroblast cells assessed as decrease in TNF- alpha induced MMP-3 mRNA level preincubated for 2 hrs followed by TNF-alpha addition for 3 hrs by qPCR analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 10. | ALA4030863 | B | Inhibition of UbcH5c in human HeLa cells assessed as TNF-alpha induced linear polyubiquitination of NEMO preincubated for 2 hrs followed by TNF-alpha addition for 5 mins by Western blot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 11. | ALA4030866 | B | Inhibition of UbcH7 (unknown origin) at 2.5 to 10 uM preincubated for 15 mins followed by E1, Ub and ATP addition measured after 40 mins by Western blot analysis | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 12. | ALA4030867 | B | Inhibition of UbcH10 (unknown origin) at 2.5 to 10 uM preincubated for 15 mins followed by E1, Ub and ATP addition measured after 40 mins by Western blot analysis | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 13. | ALA4030868 | B | Inhibition of E2-25K (unknown origin) at 2.5 to 10 uM preincubated for 15 mins followed by E1, Ub and ATP addition measured after 40 mins by Western blot analysis | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 14. | ALA4030869 | B | Inhibition of UbcH2 (unknown origin) at 2.5 to 10 uM preincubated for 15 mins followed by E1, Ub and ATP addition measured after 40 mins by Western blot analysis | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 15. | ALA4030871 | B | Inhibition of UbcH5c (unknown origin) at 5 uM preincubated for 15 mins followed by E1, Ub and ATP addition measured after 40 mins by Western blot analysis | Homo sapiens | 52 | single protein format | Scientific Literature | ||
| 16. | ALA4030874 | B | Inhibition of UbcH5c in mouse b.End3 cells assessed as decrease in TNF-alpha-mediated IkB-alpha phosphorylation preincubated for 2 hrs followed by TNF-alpha stimulation for 20 mins by Western blot analysis | Mus musculus | 6 | cell-based format | Scientific Literature | ||
| 17. | ALA4030875 | B | Inhibition of UbcH5c in mouse b.End3 cells assessed as decrease in TNF-alpha-mediated IkB-alpha degradation preincubated for 2 hrs followed by TNF-alpha stimulation for 20 mins by Western blot analysis | Mus musculus | 6 | cell-based format | Scientific Literature | ||
| 18. | ALA4030876 | B | Inhibition of UbcH5c in mouse b.End3 cells assessed as decrease in TNF-alpha-mediated p65 phosphorylation preincubated for 2 hrs followed by TNF-alpha stimulation for 20 mins by Western blot analysis | Mus musculus | 6 | cell-based format | Scientific Literature | ||
| 19. | ALA4030877 | B | Inhibition of Ubc13 (unknown origin) at 2.5 to 10 uM preincubated for 20 mins followed by E1, Ub and ATP addition measured after 40 mins iby Western blot analysis | Homo sapiens | 6 | single protein format | Scientific Literature | ||
| 20. | ALA4030878 | B | Inhibition of UbcH5c (unknown origin) preincubated for 15 mins followed by E1, Ub and ATP addition measured after 40 mins by Western blot analysis | Homo sapiens | 5 | single protein format | Scientific Literature |
The page will load shortly, Thanks for your patience!