# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4021741 | Functional | Anti-inflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production by measuring remaining NO level at 20 ug/ml after 22 hrs by Griess assay relative to control | Mus musculus | 30 | ALA4020752 | cell-based format | Scientific Literature | |
2. | ALA4021743 | Functional | Anti-inflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production by measuring nitrate level at 20 ug/ml after 22 hrs by Griess assay (Rvb = 25.31 uM) | Mus musculus | 30 | ALA4020752 | cell-based format | Scientific Literature | |
3. | ALA4021746 | Functional | Anti-inflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production after 22 hrs by Griess assay | Mus musculus | 1 | ALA4020752 | cell-based format | Scientific Literature | |
4. | ALA4021747 | ADME | Cytotoxicity against mouse RAW264.7 cells assessed as survival up to 20 ug/ml after 22 hrs by MTT assay relative to control | Mus musculus | 1 | ALA4020752 | cell-based format | Scientific Literature | |
5. | ALA4021748 | ADME | Cytotoxicity against mouse RAW264.7 cells assessed as survival at 40 ug/ml after 22 hrs by MTT assay relative to control | Mus musculus | 1 | ALA4020752 | cell-based format | Scientific Literature | |
6. | ALA4021749 | Functional | Downregulation of LPS-stimulated iNOS protein expression level in mouse RAW264.7 cells preincubated for 30 mins followed by LPS stimulation for 16 hrs by Western blot analysis | Mus musculus | 1 | ALA4020752 | cell-based format | Scientific Literature | |
7. | ALA4021750 | Functional | Downregulation of LPS-stimulated iNOS mRNA expression level in mouse RAW264.7 cells preincubated for 30 mins followed by LPS stimulation for 4 hrs by RT-PCR method | Mus musculus | 1 | ALA4020752 | cell-based format | Scientific Literature | |
8. | ALA4021751 | Functional | Downregulation of LPS-stimulated TNF-alpha protein expression level in mouse RAW264.7 cells up to 20 ug/ml preincubated for 30 mins followed by LPS stimulation for 4 hrs by Western blot analysis | Mus musculus | 1 | ALA4020752 | cell-based format | Scientific Literature | |
9. | ALA4021752 | Functional | Downregulation of LPS-stimulated IL-1beta protein expression level in mouse RAW264.7 cells up to 20 ug/ml preincubated for 30 mins followed by LPS stimulation for 4 hrs by Western blot analysis | Mus musculus | 1 | ALA4020752 | cell-based format | Scientific Literature | |
10. | ALA4021753 | Functional | Downregulation of LPS-stimulated IL-1beta mRNA expression level in mouse RAW264.7 cells preincubated for 30 mins followed by LPS stimulation for 4 hrs by RT-PCR method | Mus musculus | 1 | ALA4020752 | cell-based format | Scientific Literature | |
11. | ALA4021754 | Binding | Inhibition of NF-kB transcriptional activity in LPS-stimulated mouse RAW264.7 cells up to 20 ug/ml preincubated for 2 hrs followed by LPS stimulation for 16 hrs by SEAP reporter gene assay | Mus musculus | 1 | ALA4020752 | assay format | Scientific Literature | |
12. | ALA4021755 | Binding | Inhibition of IkB kinase in LPS-stimulated mouse RAW264.7 cells assessed as reduction in IkB-alpha phosphorylation up to 20 ug/ml preincubated with cells followed by LPS stimulation for 30 mins by Western blot analysis | Mus musculus | 1 | ALA4020752 | assay format | Scientific Literature | |
13. | ALA4021756 | Functional | Downregulation of IkB-alpha level in LPS-stimulated mouse RAW264.7 cells up to 20 ug/ml preincubated with cells followed by LPS stimulation for 30 mins by Western blot analysis | Mus musculus | 1 | ALA4020752 | cell-based format | Scientific Literature | |
14. | ALA4021757 | Functional | Downregulation of IKKbeta level in LPS-stimulated mouse RAW264.7 cells up to 20 ug/ml preincubated with cells followed by LPS stimulation for 16 hrs by Western blot analysis | Mus musculus | 1 | ALA4020752 | cell-based format | Scientific Literature | |
15. | ALA4021758 | Binding | Inhibition of LPS-stimulated IRF-3 phosphorylation at Ser-396 residue in mouse RAW264.7 cells up to 20 ug/ml preincubated for 30 mins followed by LPS stimulation for 30 mins by Western blot analysis | Mus musculus | 1 | ALA4020752 | cell-based format | Scientific Literature | |
16. | ALA4021759 | Functional | Downregulation of IFN-beta mRNA level in LPS-stimulated mouse RAW264.7 cells up to 20 ug/ml preincubated with cells followed by LPS stimulation for 4 hrs by RT-PCR method | Mus musculus | 1 | ALA4020752 | cell-based format | Scientific Literature | |
17. | ALA4021760 | Functional | Upregulation of HO-1 protein level in LPS-stimulated mouse RAW264.7 cells at 20 ug/ml preincubated for 30 mins followed by LPS stimulation for 16 hrs by Western blot method | Mus musculus | 1 | ALA4020752 | cell-based format | Scientific Literature |