# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA3998921 | B | Inhibition of recombinant human C-terminal FLAg-tagged ATX lysophospholipase D activity expressed in baculovirus infected sf9 cells using FRET based FS-3 substrate measured every 2 mins for 1 hr by fluorescence assay | Homo sapiens | 30 | ALA3997796 | cell-based format | Scientific Literature | |
2. | ALA3998922 | B | Inhibition of recombinant human C-terminal FLAg-tagged ATX nucleotide pyrophosphate phosphatase activity expressed in baculovirus infected sf9 cells using pNP-TMP as substrate measured every 2 mins for 1 hr by fluorescence assay | Homo sapiens | 30 | ALA3997796 | cell-based format | Scientific Literature | |
3. | ALA3998923 | B | Competitive inhibition of recombinant human C-terminal FLAg-tagged ATX expressed in baculovirus infected sf9 cells using varying levels of FRET based FS-3 substrate at 0.5 to 2 times IC50 measured every 2 mins for 1 hr by Michaelis-Menten plot analysis | Homo sapiens | 6 | ALA3997796 | cell-based format | Scientific Literature | |
4. | ALA3998924 | F | Agonist activity at LPA1 receptor (unknown origin) expressed in rat RH7777 cells assessed as increase in intracellular calcium level up to 10 uM measured every 3.42 secs for 70 secs by Fura-2-AM dye based fluorescence assay | Homo sapiens | 3 | ALA3997796 | cell-based format | Scientific Literature | |
5. | ALA3998925 | F | Agonist activity at LPA3 receptor (unknown origin) expressed in rat RH7777 cells assessed as increase in intracellular calcium level up to 10 uM measured every 3.42 secs for 70 secs by Fura-2-AM dye based fluorescence assay | Homo sapiens | 3 | ALA3997796 | cell-based format | Scientific Literature | |
6. | ALA3998926 | F | Agonist activity at LPA2 receptor (unknown origin) expressed in MEF cells assessed as increase in intracellular calcium level up to 10 uM measured every 3.42 secs for 70 secs by Fura-2-AM dye based fluorescence assay | Homo sapiens | 3 | ALA3997796 | cell-based format | Scientific Literature | |
7. | ALA3998927 | F | Agonist activity at LPA4 receptor (unknown origin) expressed in CHO cells assessed as increase in intracellular calcium level up to 10 uM measured every 3.42 secs for 70 secs by Fura-2-AM dye based fluorescence assay | Homo sapiens | 3 | ALA3997796 | cell-based format | Scientific Literature | |
8. | ALA3998928 | F | Agonist activity at FLAG-tagged LPA5 receptor (unknown origin) expressed in rat B103 cells assessed as increase in intracellular calcium level up to 10 uM measured every 3.42 secs for 70 secs by Fura-2-AM dye based fluorescence assay | Homo sapiens | 3 | ALA3997796 | cell-based format | Scientific Literature | |
9. | ALA3998929 | F | Antagonist activity at LPA1 receptor (unknown origin) expressed in rat RH7777 cells assessed as inhibition of LPA-induced intracellular calcium level up to 100 uM measured every 3.42 secs for 70 secs by Fura-2-AM dye based fluorescence assay relative to LPA | Homo sapiens | 5 | ALA3997796 | cell-based format | Scientific Literature | |
10. | ALA3998930 | F | Antagonist activity at LPA1 receptor (unknown origin) expressed in rat RH7777 cells assessed as inhibition of LPA-induced intracellular calcium level measured every 3.42 secs for 70 secs by Fura-2-AM dye based fluorescence assay | Homo sapiens | 5 | ALA3997796 | cell-based format | Scientific Literature | |
11. | ALA3998931 | F | Antagonist activity at LPA3 receptor (unknown origin) expressed in rat RH7777 cells assessed as inhibition of LPA-induced intracellular calcium level up to 100 uM measured every 3.42 secs for 70 secs by Fura-2-AM dye based fluorescence assay | Homo sapiens | 5 | ALA3997796 | cell-based format | Scientific Literature | |
12. | ALA3998932 | F | Antagonist activity at LPA2 receptor (unknown origin) expressed in MEF cells assessed as inhibition of LPA-induced intracellular calcium level up to 100 uM measured every 3.42 secs for 70 secs by Fura-2-AM dye based fluorescence assay | Homo sapiens | 5 | ALA3997796 | cell-based format | Scientific Literature | |
13. | ALA3998933 | F | Antagonist activity at LPA4 receptor (unknown origin) expressed in CHO cells assessed as inhibition of LPA-induced intracellular calcium level up to 100 uM measured every 3.42 secs for 70 secs by Fura-2-AM dye based fluorescence assay | Homo sapiens | 5 | ALA3997796 | cell-based format | Scientific Literature | |
14. | ALA3998934 | F | Antagonist activity at FLAG-tagged LPA5 receptor (unknown origin) expressed in rat B103 cells assessed as inhibition of LPA-induced intracellular calcium level up to 100 uM measured every 3.42 secs for 70 secs by Fura-2-AM dye based fluorescence assay | Homo sapiens | 5 | ALA3997796 | cell-based format | Scientific Literature | |
15. | ALA3998935 | B | Inhibition of ENPP6 (unknown origin) using pNPPC as substrate measured every 2 mins for 1 hr by fluorescence assay | Homo sapiens | 30 | ALA3997796 | single protein format | Scientific Literature | |
16. | ALA3998936 | B | Inhibition of ENPP7 (unknown origin) using pNPPC as substrate measured every 2 mins for 1 hr by fluorescence assay | Homo sapiens | 30 | ALA3997796 | single protein format | Scientific Literature | |
17. | ALA3998937 | B | Inhibition of ATX (unknown origin) expressed in human A2058 cells assessed as inhibition of LPC-induced cell invasion after 20 hrs by calcein-AM dye based matrigel-mediated Boyden chamber assay | Homo sapiens | 4 | ALA3997796 | cell-based format | Scientific Literature | |
18. | ALA3998938 | B | Inhibition of ATX (unknown origin) expressed in human A2780 cells assessed as inhibition of LPC-induced cell invasion at 10 uM after 20 hrs by calcein-AM dye based matrigel-mediated Boyden chamber assay | Homo sapiens | 5 | ALA3997796 | cell-based format | Scientific Literature | |
19. | ALA3998940 | A | Stability in mouse 4T1 cells assessed as parent compound remaining at 10 uM after 16 hrs by LC-MS/MS method | Mus musculus | 4 | ALA3997796 | cell-based format | Scientific Literature | |
20. | ALA3998941 | A | Stability in mouse 4T1 cells assessed as parent compound remaining at 10 uM after 40 hrs by LC-MS/MS method | Mus musculus | 4 | ALA3997796 | cell-based format | Scientific Literature |