#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4053981	B	Inhibition of chymotrypsin like activity of 26S proteasome beta 5 subunit in human U266 cells assessed as residual activity using N-Suc-LLVY-AMC as substrate at 10 uM preincubated for 1 hr followed by substrate addition and measured after 4 hrs by fluorescence assay relative to control	Homo sapiens	19		cell-based format	Scientific Literature
2.	ALA4053982	F	Antiproliferative activity against human K562 cells after 72 hrs by calcein AM staining based fluorescence assay	Homo sapiens	47		cell-based format	Scientific Literature
3.	ALA4053983	F	Antiproliferative activity against human CEM cells after 72 hrs by calcein AM staining based fluorescence assay	Homo sapiens	47		cell-based format	Scientific Literature
4.	ALA4053984	F	Antiproliferative activity against human U266 cells after 72 hrs by calcein AM staining based fluorescence assay	Homo sapiens	47		cell-based format	Scientific Literature
5.	ALA4053985	B	Inhibition of chymotrypsin-like activity of 26S proteasome beta 5 subunit in human U266 cells using Suc-LLVY-aminoluciferin as substrate after 1 hr by Proteosome-Glo luminescence assay	Homo sapiens	47		cell-based format	Scientific Literature
6.	ALA4053986	B	Inhibition of trypsin like activity of 26S proteasome beta 2 subunit in human U266 cells assessed as residual activity using Z-LLE-AMC as substrate at 10 uM preincubated for 1 hr followed by substrate addition and measured after 4 hrs by fluorescence assay relative to control	Homo sapiens	19		cell-based format	Scientific Literature
7.	ALA4053987	B	Inhibition of proteasome in human U2OS cells stably expressing GFP fused to short degron assessed as increase in GFP accumulation pre-incubated for 1 hr and measured after 9 hrs by live-cell imaging microscopy	Homo sapiens	8		cell-based format	Scientific Literature
8.	ALA4053988	B	Inhibition of proteasome in human U2OS cells stably expressing GFP fused to short degron assessed as increase in GFP accumulation at 200 nM pre-incubated for 1 hr and measured after 9 hrs by live-cell imaging microscopy	Homo sapiens	1		cell-based format	Scientific Literature
9.	ALA4053989	A	Cytotoxicity against human U2OS cells at 6.25 uM after 16 hrs by live-cell imaging microscopic analysis	Homo sapiens	1		cell-based format	Scientific Literature
10.	ALA4053990	B	Inhibition of caspase like activity of 26S proteasome beta 1 subunit in human U266 cells assessed as residual activity using Bz-VGR-AMC as substrate at 10 uM preincubated for 1 hr followed by substrate addition and measured after 4 hrs by fluorescence assay relative to control	Homo sapiens	19		cell-based format	Scientific Literature
11.	ALA4053991	F	Antiproliferative activity against human U2OS cells up to 1 uM after 24 hrs by live-cell imaging microscopic analysis	Homo sapiens	1		cell-based format	Scientific Literature
12.	ALA4053992	F	Antiproliferative activity against human U2OS cells up to 1 uM after 48 hrs by live-cell imaging microscopic analysis	Homo sapiens	1		cell-based format	Scientific Literature
13.	ALA4053993	F	Antiproliferative activity against human U2OS cells at 200 nM after 72 hrs by live-cell imaging microscopic analysis	Homo sapiens	1		cell-based format	Scientific Literature
14.	ALA4053994	B	Inhibition of proteasome in human U266 cells assessed as increase in accumulation of polyubiquitinated proteins at 250 nM by Western blot analysis	Homo sapiens	1		cell-based format	Scientific Literature
15.	ALA4053995	B	Inhibition of proteasome in human U266 cells assessed as increase in p27KIP level up to 24 hrs by Western blot method	Homo sapiens	1		cell-based format	Scientific Literature
16.	ALA4053996	F	Induction of apoptosis in human U266 cells assessed as increase in caspase-3/7 activity using Ac-DEVD-AMC as substrate at 250 nM by fluorescence assay	Homo sapiens	1		cell-based format	Scientific Literature
17.	ALA4053997	F	Induction of apoptosis in human U266 cells assessed as PARP1 cleavage at 250 nM after 16 to 24 hrs by Western blot method	Homo sapiens	1		cell-based format	Scientific Literature
18.	ALA4053998	F	Induction of apoptosis in human U266 cells assessed as caspase-7 cleavage at 250 nM after 16 to 24 hrs by Western blot method	Homo sapiens	1		cell-based format	Scientific Literature
19.	ALA4053999	F	Induction of apoptosis in human U266 cells assessed as downregulation of Mcl-1 level at 250 nM after 16 to 24 hrs by Western blot method	Homo sapiens	1		cell-based format	Scientific Literature
20.	ALA4054000	A	Effect on beta-actin protein levels in human U266 cells at 250 nM after 2 to 24 hrs by Western blot analysis	Homo sapiens	1		cell-based format	Scientific Literature