# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4042745 | Binding | Inhibition of kappaB DNA binding to NFkappaB p65 in LPS-stimulated mouse RAW264.7 nuclear extract at 3 to 10 ug/ml after 15 mins by colorimetric method | Mus musculus | 2 | ALA4041535 | single protein format | Scientific Literature | |
2. | ALA4042746 | ADME | Cytotoxicity against mouse RAW264.7 cells assessed as reduction in cell viability up to 1 ug/ml after 24 hrs by MTT assay | Mus musculus | 1 | ALA4041535 | cell-based format | Scientific Literature | |
3. | ALA4042747 | Binding | Inhibition of LPS-induced NFkappaB p65 activation in mouse RAW264.7 cells at 0.3 to 1 ug/ml preincubated for 30 mins followed by LPS addition measured after 2 hrs by kappaB DNA binding based colorimetric method | Mus musculus | 2 | ALA4041535 | cell-based format | Scientific Literature | |
4. | ALA4042748 | Binding | Inhibition of LPS-induced NFkappaB p65-mediated iNOS induction in mouse RAW264.7 cells assessed as reduction in NO production preincubated for 30 mins followed by LPS addition measured after 24 hrs by Griess assay | Mus musculus | 1 | ALA4041535 | cell-based format | Scientific Literature | |
5. | ALA4042750 | Binding | Inhibition of LPS-induced NFkappaB p65-mediated iNOS mRNA expression in mouse RAW264.7 cells at 0.1 to 1 ug/ml preincubated for 30 mins followed by LPS addition measured after 24 hrs by qPCR method | Mus musculus | 1 | ALA4041535 | cell-based format | Scientific Literature | |
6. | ALA4042751 | Binding | Inhibition of LPS-induced NFkappaB p65-mediated IL-6 secretion in mouse RAW264.7 cells at 0.1 to 1 ug/ml preincubated for 30 mins followed by LPS addition measured after 24 hrs by ELISA | Mus musculus | 1 | ALA4041535 | cell-based format | Scientific Literature | |
7. | ALA4042752 | Binding | Inhibition of LPS-induced NFkappaB p65-mediated IL-1beta secretion in mouse RAW264.7 cells at 0.1 to 1 ug/ml preincubated for 30 mins followed by LPS addition measured after 24 hrs by ELISA | Mus musculus | 1 | ALA4041535 | cell-based format | Scientific Literature | |
8. | ALA4042753 | Binding | Inhibition of LPS-induced NFkappaB p65-mediated TNF-alpha secretion in mouse RAW264.7 cells at 0.1 to 1 ug/ml preincubated for 30 mins followed by LPS addition measured after 24 hrs by ELISA | Mus musculus | 1 | ALA4041535 | cell-based format | Scientific Literature | |
9. | ALA4042754 | Binding | Inhibition of kappaB DNA binding to NFkappaB p65 in human ES2 nuclear extract at 3 to 10 ug/ml after 15 mins by colorimetric method | Homo sapiens | 1 | ALA4041535 | single protein format | Scientific Literature | |
10. | ALA4042755 | ADME | Cytotoxicity against human ES2 cells assessed as reduction in cell viability up to 30 ug/ml after 24 hrs by MTT assay | Homo sapiens | 1 | ALA4041535 | cell-based format | Scientific Literature | |
11. | ALA4042756 | Binding | Inhibition of constitutively activated NFkappaB p65 in human ES2 cells at 3 to 10 ug/ml after 2 hrs by kappaB DNA binding based colorimetric method | Homo sapiens | 2 | ALA4041535 | cell-based format | Scientific Literature | |
12. | ALA4042757 | Functional | Antimigratory activity against human ES2 cells at 1 to 10 ug/ml after 24 hrs by radius 96-well migration assay | Homo sapiens | 1 | ALA4041535 | cell-based format | Scientific Literature | |
13. | ALA4042758 | Functional | Anti-invasive activity against human ES2 cells at 0.1 to 0.3 ug/ml after 24 hrs by Diff-Quick staining based matrigel chamber assay | Homo sapiens | 1 | ALA4041535 | cell-based format | Scientific Literature | |
14. | ALA4042760 | Binding | Inhibition of NFkappaB p65 in human ES2 cells assessed as reduction in MMP2 secretion at 3 to 10 ug/ml after 24 hrs by ELISA | Homo sapiens | 1 | ALA4041535 | cell-based format | Scientific Literature | |
15. | ALA4042761 | Binding | Inhibition of NFkappaB p65 in human ES2 cells assessed as reduction in MMP2 mRNA expression at 0.1 to 1 ug/ml after 24 hrs by qPCR method | Homo sapiens | 1 | ALA4041535 | cell-based format | Scientific Literature | |
16. | ALA4042762 | Physicochemical | Chemical stability in PBS at 100 ug/ml up to 12 hrs at 37 degC by HPLC analysis | 2 | ALA4041535 | small-molecule physicochemical format | Scientific Literature | ||
17. | ALA4042763 | Binding | Inhibition of LPS-induced NFkappaB p65-mediated iNOS induction in mouse RAW264.7 cells assessed as reduction in NO production at 1 ug/ml using 12 hrs PBS incubated compound preincubated for 30 mins with cells followed by LPS addition measured after 24 hrs by Griess assay | Mus musculus | 1 | ALA4041535 | cell-based format | Scientific Literature |