#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4027106	Functional	Blockade of nicotine self administration in Sprague-Dawley rat assessed as decrease in cumulative nicotine infusion rate at 0.3 mg/kg, sc administered 10 mins prior to beginning of the session measured after 2 hrs	Rattus norvegicus	1	ALA4024764	organism-based format	Scientific Literature
2.	ALA4027107	Functional	Blockade of nicotine self administration in Sprague-Dawley rat assessed as decrease in cumulative nicotine infusion rate at 1 mg/kg, sc administered 10 mins prior to beginning of the session measured after 2 hrs	Rattus norvegicus	1	ALA4024764	organism-based format	Scientific Literature
3.	ALA4027108	Functional	Blockade of nicotine self administration in Sprague-Dawley rat at 0.3 to 1 mg/kg, sc administered 10 mins prior to beginning of the session measured for 30 mins	Rattus norvegicus	1	ALA4024764	organism-based format	Scientific Literature
4.	ALA4027109	Binding	Displacement of [3H]epibatidine from rat alpha3beta4 nAChR expressed in HEK cell membranes after 2 hrs	Rattus norvegicus	23	ALA4024764	cell-based format	Scientific Literature
5.	ALA4027110	Binding	Displacement of [3H]epibatidine from rat alpha4beta4 nAChR expressed in HEK cell membranes after 2 hrs	Rattus norvegicus	2	ALA4024764	cell-based format	Scientific Literature
6.	ALA4027111	Binding	Displacement of [3H]epibatidine from rat alpha4beta2 nAChR expressed in HEK cell membranes after 2 hrs	Rattus norvegicus	26	ALA4024764	cell-based format	Scientific Literature
7.	ALA4027112	Binding	Displacement of [3H]epibatidine from rat alpha3beta2 nAChR expressed in HEK cell membranes after 2 hrs	Rattus norvegicus	2	ALA4024764	cell-based format	Scientific Literature
8.	ALA4027113	Binding	Displacement of [3H]epibatidine from rat alpha3beta4alpha5 nAChR expressed in HEK cells after 2 hrs	Rattus norvegicus	2	ALA4024764	cell-based format	Scientific Literature
9.	ALA4027114	Binding	Selectivity ratio of Ki for rat alpha3beta4 nAChR expressed in HEK cells to Ki for rat alpha4beta2 nAChR expressed in HEK cells	Rattus norvegicus	23	ALA4024764	cell-based format	Scientific Literature
10.	ALA4027115	Binding	Selectivity ratio of Ki for rat alpha4beta4 nAChR expressed in HEK cells to Ki for rat alpha4beta2 nAChR expressed in HEK cells	Rattus norvegicus	2	ALA4024764	cell-based format	Scientific Literature
11.	ALA4027116	Binding	Selectivity ratio of Ki for rat alpha3beta4alpha5 nAChR expressed in HEK cells to Ki for rat alpha4beta2 nAChR expressed in HEK cells	Rattus norvegicus	2	ALA4024764	cell-based format	Scientific Literature
12.	ALA4027117	Binding	Antagonist activity at rat alpha4beta2 nAChR expressed in HEK cells assessed as inhibition of epibatidine-induced membrane potential preincubated for 10 mins followed by epibatidine addition by fluorescence assay	Rattus norvegicus	2	ALA4024764	cell-based format	Scientific Literature
13.	ALA4027118	Binding	Antagonist activity at rat alpha3beta4 nAChR expressed in HEK cells assessed as inhibition of Ca2+ flux	Rattus norvegicus	2	ALA4024764	cell-based format	Scientific Literature
14.	ALA4027119	Binding	Agonist activity at rat alpha3beta4 nAChR expressed in HEK cells assessed as induction of Ca2+ flux	Rattus norvegicus	2	ALA4024764	cell-based format	Scientific Literature
15.	ALA4027120	Functional	Reduction in nicotine priming-induced reinstatement of nicotine seeking behavior in Sprague-Dawley rat at 0.3 to 1 mg/kg, sc administered 10 mins prior to nicotine injection and 1 hr prior to reinstatement session measured for 60 mins	Rattus norvegicus	1	ALA4024764	organism-based format	Scientific Literature
16.	ALA4027121	Functional	Reduction in cue-induced reinstatement of nicotine seeking behavior in Sprague-Dawley rat at 1 mg/kg, sc administered 10 mins prior to onset of reinstatement session measured for 60 mins	Rattus norvegicus	1	ALA4024764	organism-based format	Scientific Literature
17.	ALA4027122	Toxicity	Toxicity in Sprague-Dawley rat assessed as induction of hypothermia at 1 mg/kg, sc after 15 to 120 mins	Rattus norvegicus	1	ALA4024764	organism-based format	Scientific Literature
18.	ALA4027123	Functional	Blockade of nicotine induced hypothermia in Sprague-Dawley rat at 1 mg/kg, sc administered 10 mins followed by nicotine challenge measured after 15 to 120 mins	Rattus norvegicus	1	ALA4024764	organism-based format	Scientific Literature
19.	ALA4027124	ADME	Drug uptake in Sprague-Dawley rat blood at 2 mg/kg, sc after 10 mins by LC-MS/MS analysis	Rattus norvegicus	1	ALA4024764	organism-based format	Scientific Literature
20.	ALA4027125	ADME	Drug uptake in Sprague-Dawley rat brain at 2 mg/kg, sc after 10 mins by LC-MS/MS analysis	Rattus norvegicus	1	ALA4024764	organism-based format	Scientific Literature