# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4054003 | B | Inhibition of Pseudomonas aeruginosa 301-5473 6His-tagged VIM-2 (V27 to E268 residues) expressed in Escherichia coli BL21 Star(DE3) pLysS using nitrocefin as substrate preincubated for 5 mins followed by substrate addition by spectrophotometric analysis | Pseudomonas aeruginosa | 23 | assay format | Scientific Literature | ||
2. | ALA4054004 | B | Inhibition of Pseudomonas aeruginosa 6His-tagged GIM-1 (Q19 to D250 residues) expressed in Escherichia coli BL21 Star(DE3) pLysS using nitrocefin as substrate preincubated for 5 mins followed by substrate addition by spectrophotometric analysis | Pseudomonas aeruginosa | 23 | assay format | Scientific Literature | ||
3. | ALA4054005 | B | Inhibition of Klebsiella pneumoniae 6His-tagged NDM1 (G36 to R280 residues) expressed in Escherichia coli BL21 Star(DE3) pLysS using imipenem as substrate preincubated for 5 mins followed by substrate addition by spectrophotometric analysis | Klebsiella pneumoniae | 20 | assay format | Scientific Literature | ||
4. | ALA4054006 | B | Inhibition of Pseudomonas aeruginosa 301-5473 VIM-2 expressed in Escherichia coli SNO3 whole cells using nitrocefin as substrate at 250 uM preincubated for 20 mins followed by substrate addition measured every min up to 60 mins by spectrophotometric analysis relative to control | Pseudomonas aeruginosa | 21 | cell-based format | Scientific Literature | ||
5. | ALA4054007 | B | Inhibition of Pseudomonas aeruginosa GIM-1 expressed in Escherichia coli SNO3 whole cells using nitrocefin as substrate at 250 uM preincubated for 20 mins followed by substrate addition measured every min up to 60 mins by spectrophotometric analysis relative to control | Pseudomonas aeruginosa | 21 | cell-based format | Scientific Literature | ||
6. | ALA4054008 | B | Inhibition of Klebsiella pneumoniae NDM1 expressed in Escherichia coli SNO3 whole cells using imipenem as substrate at 250 uM preincubated for 20 mins followed by substrate addition measured every min up to 60 mins by spectrophotometric analysis relative to control | Klebsiella pneumoniae | 20 | cell-based format | Scientific Literature |