#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3995661	B	Inhibition of human recombinant topoisomerase-2alpha-mediated relaxation of supercoiled pBR322 DNA at 100 uM after 30 mins by ethidium bromide staining based agarose gel electrophoresis method relative to control	Homo sapiens	19	ALA3994616	single protein format	Scientific Literature
2.	ALA3995662	B	Inhibition of human recombinant topoisomerase-alpha-mediated relaxation of supercoiled pBR322 DNA at 20 uM after 30 mins by ethidium bromide staining based agarose gel electrophoresis method relative to control	Homo sapiens	19	ALA3994616	single protein format	Scientific Literature
3.	ALA3995663	B	Inhibition of human recombinant topoisomerase 1-mediated relaxation of supercoiled pBR322 DNA at 100 uM after 30 mins by ethidium bromide staining based agarose gel electrophoresis method relative to control	Homo sapiens	19	ALA3994616	single protein format	Scientific Literature
4.	ALA3995664	B	Inhibition of human recombinant topoisomerase 1-mediated relaxation of supercoiled pBR322 DNA at 20 uM after 30 mins by ethidium bromide staining based agarose gel electrophoresis method relative to control	Homo sapiens	19	ALA3994616	single protein format	Scientific Literature
5.	ALA3995665	F	Antiproliferative activity against human HCT15 cells after 72 hrs by CCK8 assay	Homo sapiens	21	ALA3994616	cell-based format	Scientific Literature
6.	ALA3995666	F	Antiproliferative activity against human T47D cells after 72 hrs by CCK8 assay	Homo sapiens	21	ALA3994616	cell-based format	Scientific Literature
7.	ALA3995667	F	Antiproliferative activity against human HeLa cells after 72 hrs by CCK8 assay	Homo sapiens	21	ALA3994616	cell-based format	Scientific Literature
8.	ALA3995671	B	Inhibition of human recombinant topoisomerase 2 alpha-mediated relaxation of supercoiled pBR322 DNA at 100 uM after 30 mins by ethidium bromide staining based agarose gel electrophoresis method relative to etoposide	Homo sapiens	28	ALA3994616	single protein format	Scientific Literature
9.	ALA3995672	B	Inhibition of human recombinant topoisomerase 2 alpha-mediated relaxation of supercoiled pBR322 DNA at 20 uM after 30 mins by ethidium bromide staining based agarose gel electrophoresis method relative to etoposide	Homo sapiens	28	ALA3994616	single protein format	Scientific Literature
10.	ALA3995673	B	Inhibition of human recombinant topoisomerase 1-mediated relaxation of supercoiled pBR322 DNA at 100 uM after 30 mins by ethidium bromide staining based agarose gel electrophoresis method relative to camptothecin	Homo sapiens	28	ALA3994616	single protein format	Scientific Literature
11.	ALA3995674	B	Inhibition of human recombinant topoisomerase 1-mediated relaxation of supercoiled pBR322 DNA at 20 uM after 30 mins by ethidium bromide staining based agarose gel electrophoresis method relative to camptothecin	Homo sapiens	28	ALA3994616	single protein format	Scientific Literature
12.	ALA3995676	B	Poison activity at human recombinant topoisomerase 2 alpha assessed as stabilization of enzyme-DNA cleavable complex by measuring formation of linear DNA at 100 to 500 uM using supercoiled pBR322 DNA after 30 mins by ethidium bromide staining based agarose gel electrophoresis method	Homo sapiens	1	ALA3994616	single protein format	Scientific Literature
13.	ALA3995677	B	Inhibition of human topoisomerase-2 alpha-mediated kinetoplast DNA decatenation after 30 mins by ethidium bromide staining based agarose gel electrophoresis	Homo sapiens	1	ALA3994616	single protein format	Scientific Literature
14.	ALA3995679	B	Poison activity at human recombinant topoisomerase 2 in human HCT15 cells assessed as induction of enzyme-DNA covalent complex by measuring increase in free enzyme at 50 uM after 2 hrs by Western blot method	Homo sapiens	2	ALA3994616	cell-based format	Scientific Literature
15.	ALA3995680	F	Induction of DNA damage in human HCT15 cells assessed as comet tail formation at 30 uM after 24 hrs using SYBRGreen stain by electrophoresis based fluorescence microscopic method (Rvb = 3 +/- 1.1%)	Homo sapiens	3	ALA3994616	cell-based format	Scientific Literature
16.	ALA3995682	F	Induction of DNA damage in human HCT15 cells assessed as increase in gammaH2AX level at 30 uM after 24 hrs by Western blot method	Homo sapiens	3	ALA3994616	cell-based format	Scientific Literature
17.	ALA3995684	B	Induction of calf thymus DNA intercalation assessed as inhibition of ethidium bromide binding to DNA up to 40 uM after 30 mins by fluorescence assay	Bos taurus	1	ALA3994616	assay format	Scientific Literature
18.	ALA3995685	B	Inhibition of topoisomerase-1/topoisomerase-2 in human HCT15 cell nuclear extract at 25 uM preincubated for 24 hrs followed by supercoiled pBR322 DNA addition measured after 20 mins by ethidium bromide staining based agarose gel electrophoresis method relative to control	Homo sapiens	3	ALA3994616	protein format	Scientific Literature
19.	ALA3995686	B	Inhibition of topoisomerase-1/topoisomerase-2 in human HCT15 cell nuclear extract at 50 uM preincubated for 24 hrs followed by supercoiled pBR322 DNA addition measured after 20 mins by ethidium bromide staining based agarose gel electrophoresis method relative to control	Homo sapiens	3	ALA3994616	protein format	Scientific Literature
20.	ALA3995687	B	Inhibition of topoisomerase-1/topoisomerase-2 in human HCT15 cell nuclear extract at 100 uM preincubated for 24 hrs followed by supercoiled pBR322 DNA addition measured after 20 mins by ethidium bromide staining based agarose gel electrophoresis method relative to control	Homo sapiens	1	ALA3994616	protein format	Scientific Literature