# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4003355 | Binding | Inhibition of human DYRK1A preincubated for 10 mins followed by YRASPSRPESPRPPA-amide substrate addition by pyruvate kinase and lactate dehydrogenase coupled enzyme assay | Homo sapiens | 1 | ALA4002585 | single protein format | Scientific Literature | |
2. | ALA4003366 | Binding | Inhibition of human CLK1 (148 to 484 residues) expressed in Escherichia coli BL21(DE3) preincubated for 10 mins followed by AFRREWSPGKEAKK substrate addition by pyruvate kinase and lactate dehydrogenase coupled enzyme assay | Homo sapiens | 2 | ALA4002585 | assay format | Scientific Literature | |
3. | ALA4003367 | Binding | Inhibition of human CLK3 (275 to 632 residues) expressed in Escherichia coli BL21(DE3) preincubated for 10 mins followed by substrate addition by pyruvate kinase and lactate dehydrogenase coupled enzyme assay | Homo sapiens | 2 | ALA4002585 | assay format | Scientific Literature | |
4. | ALA4728312 | Binding | Selective index, difference between deltaTm for recombinant wild type CLK1 (H148 to I484 residues) (unknown origin) expressed in Escherichia coli to deltaTm for recombinant CLK1 V324A mutant (unknown origin) expressed in Escherichia coli | Homo sapiens | 18 | ALA4725345 | assay format | Scientific Literature | |
5. | ALA4728313 | Binding | Selective index, difference between deltaTm for CLK3 A319V mutant (unknown origin) expressed in Escherichia coli to deltaTm for recombinant wild type CLK3 (R134 to T484 residues) (unknown origin) expressed in Escherichia coli | Homo sapiens | 18 | ALA4725345 | assay format | Scientific Literature | |
6. | ALA4728316 | Binding | Selectivity ratio of Ki for CLK1 V324A mutant (unknown origin) expressed in HEK293 cells to Ki for wild type CLK1 (unknown origin) | Homo sapiens | 4 | ALA4725345 | cell-based format | Scientific Literature |