# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4015669 | Binding | Inhibition of full length His-tagged human DNMT1 using SAM/[methyl-3H]SAM assessed as reduction of methylation in biotinylated hemimethylated DNA duplex after 1 hr by scintillation counting method | Homo sapiens | 11 | ALA4014314 | single protein format | Scientific Literature | |
2. | ALA4015670 | Binding | Inhibition of human DNMT3A catalytic domain using AdoMet assessed as reduction of methylation in 5'-biotin/3' 6-carboxyfluorescein labeled DNA duplex at CpG site after 1 hr by fluorescence assay | Homo sapiens | 11 | ALA4014314 | single protein format | Scientific Literature | |
3. | ALA4015671 | Binding | Inhibition of human DNMT3A catalytic domain using AdoMet assessed as reduction of methylation in 5'-biotin/3' 6-carboxyfluorescein labeled DNA duplex at CpG site at 32 uM after 1 hr by fluorescence assay relative to control | Homo sapiens | 50 | ALA4014314 | single protein format | Scientific Literature | |
4. | ALA4015672 | Binding | Inhibition of human DNMT3A catalytic domain using AdoMet assessed as reduction of methylation in 5'-biotin/3' 6-carboxyfluorescein labeled DNA duplex at CpG site at 10 uM after 1 hr by fluorescence assay relative to control | Homo sapiens | 50 | ALA4014314 | single protein format | Scientific Literature | |
5. | ALA4015673 | Binding | Inhibition of full length His-tagged human DNMT1 using SAM/[methyl-3H]SAM assessed as reduction of methylation in biotinylated hemimethylated DNA duplex at 32 uM after 1 hr by scintillation counting method relative to control | Homo sapiens | 50 | ALA4014314 | single protein format | Scientific Literature | |
6. | ALA4015674 | Binding | Inhibition of full length His-tagged human DNMT1 using SAM/[methyl-3H]SAM assessed as reduction of methylation in biotinylated hemimethylated DNA duplex at 100 uM after 1 hr by scintillation counting method relative to control | Homo sapiens | 50 | ALA4014314 | single protein format | Scientific Literature | |
7. | ALA4015675 | Binding | Inhibition of DNMT in human KG1 cells assessed as luciferase gene driven CMV promoter demethylation by measuring fold increase in luciferase reactivation at 5 uM after 24 hrs by luciferase reporter gene assay relative to control | Homo sapiens | 3 | ALA4014314 | cell-based format | Scientific Literature | |
8. | ALA4015676 | Binding | Inhibition of DNMT in human KG1 cells assessed as luciferase gene driven CMV promoter demethylation by measuring fold increase in luciferase reactivation at 1 uM after 24 hrs by luciferase reporter gene assay relative to control | Homo sapiens | 3 | ALA4014314 | cell-based format | Scientific Literature | |
9. | ALA4015691 | Physicochemical | Solubility of the compound in RPMI-1640 medium | 1 | ALA4014314 | small-molecule physicochemical format | Scientific Literature |