| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4037929 | B | Inhibition of anti-hMD2 antibody binding to C-terminal His6-tagged human MD2 expressed in Pichia pastoris GS115 at 20 uM preincubated for 2 hrs followed by antibody addition by sandwich ELISA | Homo sapiens | 4 | assay format | Scientific Literature | ||
| 2. | ALA4037930 | B | Displacement of biotinylated LPS from C-terminal His6-tagged human MD2 expressed in Pichia pastoris GS115 at 160 uM after 1.5 hrs by ELISA | Homo sapiens | 4 | assay format | Scientific Literature | ||
| 3. | ALA4037932 | B | Binding to C-terminal His6-tagged human MD2 expressed in Pichia pastoris GS115 by surface plasmon resonance assay | Homo sapiens | 4 | assay format | Scientific Literature | ||
| 4. | ALA4037935 | A | Cytotoxicity against HEK-Blue cells assessed as reduction in cell viability at 10 uM after overnight incubation by MTT assay | Homo sapiens | 3 | cell-based format | Scientific Literature | ||
| 5. | ALA4037936 | A | Cytotoxicity against human THP1 cells assessed as reduction in cell viability up to 10 uM after 24 hrs in presence of LPS by MTT asssay | Homo sapiens | 4 | cell-based format | Scientific Literature | ||
| 6. | ALA4037937 | B | Inhibition of TLR4/MD2 interaction in human THP1 cells assessed as reduction of LPS-induced IL8 production at 10 uM preincubated for 1 hr followed by LPS stimulation measured after 16 hrs by ELISA | Homo sapiens | 4 | cell-based format | Scientific Literature | ||
| 7. | ALA4037943 | B | Displacement of bis-ANS from C-terminal His6-tagged human MD2 expressed in Pichia pastoris GS115 up to 30 uM by fluorescence spectroscopic assay | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 8. | ALA4037944 | A | Agonist activity at human TLR4 expressed in mouse RAW blue cells assessed as increase in NF-kappaB activation-mediated SEAP production up to 10 uM measured after 20 hrs by SEAP reporter gene assay | Homo sapiens | 4 | cell-based format | Scientific Literature | ||
| 9. | ALA4037945 | B | Antagonist activity at human TLR4 expressed in mouse RAW blue cells assessed as inhibition of LPS-induced NF-kappaB activation-mediated SEAP production up to 10 uM preincubated for 30 mins followed by LPS addition measured after 16 hrs by NF-kappaB reporter gene assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 10. | ALA4037946 | A | Cytotoxicity against human THP1 cells assessed as reduction in cell viability up to 10 uM after 24 hrs in absence of LPS by MTT asssay | Homo sapiens | 4 | cell-based format | Scientific Literature |
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