| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4013545 | B | Inhibition of recombinant human N-terminal FLAG-tagged PRMT5 (2 to end residues) /human N-terminal His-tagged MEP50 (2 to end residues) expressed in HEK293F cells using histone H4 as substrate preincubated for 15 mins followed by substrate/S-adenosyl methionine addition measured after 60 mins by alpha LISA assay | Homo sapiens | 37 | ALA4011642 | cell-based format | Scientific Literature | |
| 2. | ALA4013547 | B | Inhibition of recombinant human N-terminal GST-tagged PRMT1 (2 to end residues) expressed in baculovirus infected Sf9 cell expression system using histone H4 as substrate in presence of [3H]-SAM after 60 mins by chemiluminescent assay | Homo sapiens | 1 | ALA4011642 | assay format | Scientific Literature | |
| 3. | ALA4013548 | B | Inhibition of human N-terminal GST-tagged PRMT3 (2 to end residues) expressed in Escherichia coli using histone H4 as substrate preincubated for 15 mins followed by substrate/S-adenosyl methionine addition measured after 60 mins by alpha LISA assay | Homo sapiens | 1 | ALA4011642 | assay format | Scientific Literature | |
| 4. | ALA4013549 | B | Inhibition of recombinant human N-terminal FLAG-tagged PRMT4 (2 to end residues) expressed in HEK293F cells using of histone H4 as substrate preincubated for 15 mins followed by substrate/S-adenosyl methionine addition measured after 60 mins by alpha LISA assay | Homo sapiens | 1 | ALA4011642 | cell-based format | Scientific Literature | |
| 5. | ALA4013550 | B | Inhibition of recombinant human N-terminal His-tagged PRMT6 (2 to 375 residues) expressed in Escherichia coli using of histone H3 as substrate preincubated for 15 mins followed by substrate/S-adenosyl methionine addition measured after 60 mins by alpha LISA assay | Homo sapiens | 1 | ALA4011642 | assay format | Scientific Literature | |
| 6. | ALA4013551 | B | Inhibition of recombinant human N-terminal His-tagged full length PRMT7 expressed in baculovirus using of histone H4 as substrate preincubated for 15 mins followed by substrate/[3H]-SAM addition measured after 240 mins | Homo sapiens | 1 | ALA4011642 | assay format | Scientific Literature | |
| 7. | ALA4013552 | B | Inhibition of recombinant human N-terminal FLAG-tagged PRMT8 (61 to 394 residues) expressed in baculovirus infected Sf9 cell expression system using of histone H3 as substrate preincubated for 15 mins followed by substrate/S-adenosyl methionine addition measured after 60 mins by alpha LISA assay | Homo sapiens | 1 | ALA4011642 | assay format | Scientific Literature | |
| 8. | ALA4013553 | B | Inhibition of DOT1L (unknown origin) using biotinylated H3K79me2 as substrate preincubated for 15 mins followed by substrate/ SAM addition measured after 30 mins by Alpha LISA assay | Homo sapiens | 1 | ALA4011642 | single protein format | Scientific Literature | |
| 9. | ALA4013554 | B | Inhibition of NSD1 (unknown origin) preincubated for 15 mins followed by [3H]-SAM addition measured after 4 hrs by liquid scintillation counting | Homo sapiens | 1 | ALA4011642 | single protein format | Scientific Literature | |
| 10. | ALA4013555 | B | Inhibition of DNMT1 (unknown origin) using poly(dI-DC) as substrate preincubated for 15 mins followed by substrate/ [3H]-SAM addition measured after 120 mins | Homo sapiens | 1 | ALA4011642 | single protein format | Scientific Literature | |
| 11. | ALA4013556 | B | Inhibition of SET7/9 (unknown origin) after 60 mins by Alpha LISA assay | Homo sapiens | 1 | ALA4011642 | single protein format | Scientific Literature | |
| 12. | ALA4013557 | B | Inhibition of BRD4 (unknown origin) using SGRG-K(Ac)-GG-K(Ac)-GLG-K-(Ac)-GGA-K(Ac)-RHRKVGG-K(Biotin) peptide as substrate preincubated for 10 mins followed by substrate addition for 10 mins measured after 30 mins by Alpha LISA assay | Homo sapiens | 1 | ALA4011642 | single protein format | Scientific Literature | |
| 13. | ALA4013558 | B | Inhibition of GCN5 (unknown origin) using H3 as substrate and Ac-CoA as cofactor preincubated for 10 mins followed by substrate/cofactor addition measured after 30 mins by Alpha LISA assay | Homo sapiens | 1 | ALA4011642 | single protein format | Scientific Literature | |
| 14. | ALA4013559 | B | Selectivity ratio of IC50 for recombinant human N-terminal GST-tagged PRMT1 (2 to end residues) expressed in baculovirus infected Sf9 cell expression system to IC50 for recombinant human N-terminal FLAG-tagged PRMT5/human N-terminal His-tagged MEP50 expressed in HEK293F cells | Homo sapiens | 1 | ALA4011642 | cell-based format | Scientific Literature | |
| 15. | ALA4013560 | B | Selectivity ratio of IC50 for human N-terminal GST-tagged PRMT3 (2 to end residues) expressed in Escherichia coli to IC50 for human N-terminal FLAG-tagged PRMT5/human N-terminal His-tagged MEP50 expressed in HEK293F cells | Homo sapiens | 1 | ALA4011642 | cell-based format | Scientific Literature | |
| 16. | ALA4013561 | B | Selectivity ratio of IC50 for recombinant human N-terminal FLAG-tagged PRMT4 (2 to end residues) expressed in HEK293F cells to IC50 for recombinant human N-terminal FLAG-tagged PRMT5/human N-terminal His-tagged MEP50 expressed in HEK293F cells | Homo sapiens | 1 | ALA4011642 | cell-based format | Scientific Literature | |
| 17. | ALA4013562 | B | Selectivity ratio of IC50 for recombinant human N-terminal His-tagged PRMT6 (2 to 375 residues) expressed in Escherichia coli to IC50 for human N-terminal FLAG-tagged PRMT5/human N-terminal His-tagged MEP50 expressed in HEK293F cells | Homo sapiens | 1 | ALA4011642 | cell-based format | Scientific Literature | |
| 18. | ALA4013563 | B | Selectivity ratio of IC50 for recombinant human N-terminal His-tagged full length PRMT7 expressed in baculovirus to IC50 for human N-terminal FLAG-tagged PRMT5/human N-terminal His-tagged MEP50 expressed in HEK293F cells | Homo sapiens | 1 | ALA4011642 | cell-based format | Scientific Literature | |
| 19. | ALA4013564 | B | Selectivity ratio of IC50 for recombinant human N-terminal FLAG-tagged PRMT8 (61 to 394 residues) expressed in baculovirus infected Sf9 cell expression system to IC50 for human N-terminal FLAG-tagged PRMT5/human N-terminal His-tagged MEP50 expressed in HEK293F cells | Homo sapiens | 1 | ALA4011642 | cell-based format | Scientific Literature | |
| 20. | ALA4013565 | B | Selectivity ratio of IC50 for NSD1 (unknown origin) to IC50 for human N-terminal FLAG-tagged PRMT5/human N-terminal His-tagged MEP50 expressed in HEK293F cells | Homo sapiens | 1 | ALA4011642 | cell-based format | Scientific Literature |
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