# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4005963 | Binding | Stabilization of 5'Dabcyl/3'FAM labeled human telomeric sequence G-quadruplex DNA assessed as increase in melting temperature at 2.5 uM pretreated for 5 mins at 30 degreeC followed by 5 mins incubation at 95 degreeC by fluorescence assay | Homo sapiens | 13 | ALA4004831 | assay format | Scientific Literature | |
2. | ALA4005964 | Binding | Stabilization of 5'Dabcyl/3'FAM labeled duplex DNA (unknown origin) assessed as increase in melting temperature at 2.5 uM pretreated for 5 mins at 30 degreeC followed by 5 mins incubation at 95 degreeC by fluorescence assay | Not specified | 13 | ALA4004831 | assay format | Scientific Literature | |
3. | ALA4005965 | Binding | Inhibition of human topoisomerase-2alpha-mediated relaxation of supercoiled pBR322 DNA after 1 hr by ethidium bromide staining based agarose gel electrophoresis | Homo sapiens | 13 | ALA4004831 | single protein format | Scientific Literature | |
4. | ALA4005966 | Binding | Poison activity at human topoisomerase-2alpha assessed as stabilization of DNA-enzyme cleavage complex at 0.5 to 100 uM using supercoiled pBR322 DNA as substrate after 1 hr by ethidium bromide staining based agarose gel electrophoresis | Homo sapiens | 1 | ALA4004831 | single protein format | Scientific Literature | |
5. | ALA4005967 | Binding | Inhibition of Thermus aquaticus DNA polymerase-mediated amplification of fragment of pBR322 by PCR | Thermus aquaticus | 13 | ALA4004831 | assay format | Scientific Literature | |
6. | ALA4005968 | Binding | Binding affinity to Escherichia coli supercoiled pBR322 DNA assessed as DNA intercalation up to 100 uM by agarose gel electrophoresis | Escherichia coli | 2 | ALA4004831 | assay format | Scientific Literature | |
7. | ALA4005969 | Binding | Inhibition of telomerase activity in human HeLa cells using 5'AATCCGTCGAGCAGAGTT3' as substrate after 30 mins by Sybr green1 staining based TRAP-PCR method | Homo sapiens | 3 | ALA4004831 | cell-based format | Scientific Literature | |
8. | ALA4005971 | Functional | Induction of apoptosis in human Jurkat T cells at 6.4 uM after 24 hrs by annexin V-phycoerythrin/7-AAD staining based flow cytometry (Rvb = 5.8 to 6.6%) | Homo sapiens | 2 | ALA4004831 | cell-based format | Scientific Literature | |
9. | ALA4005972 | Functional | Induction of apoptosis in human Jurkat T cells at 3.2 uM after 24 hrs by annexin V-phycoerythrin/7-AAD staining based flow cytometry (Rvb = 5.8 to 6.6%) | Homo sapiens | 1 | ALA4004831 | cell-based format | Scientific Literature | |
10. | ALA4005973 | Functional | Induction of apoptosis in human Jurkat T cells assessed as caspase3 activation at 1.6 uM after 24 hrs by colorimetric assay relative to control | Homo sapiens | 1 | ALA4004831 | cell-based format | Scientific Literature | |
11. | ALA4005974 | Functional | Induction of apoptosis in human Jurkat T cells assessed as caspase3 activation at 6.4 uM after 24 hrs by colorimetric assay relative to control | Homo sapiens | 1 | ALA4004831 | cell-based format | Scientific Literature | |
12. | ALA4005975 | Functional | Induction of apoptosis in human Jurkat T cells assessed as PARP cleavage at 3.2 uM after 24 hrs by flow cytometry (Rvb = 4.8%) | Homo sapiens | 2 | ALA4004831 | cell-based format | Scientific Literature | |
13. | ALA4005976 | Functional | Cell cycle arrest in human Jurkat T cells assessed as accumulation at G2/M phase at 3.2 uM after 24 hrs by propidium iodide staining based flow cytometry (Rvb = 17.1 to 20.8%) | Homo sapiens | 2 | ALA4004831 | cell-based format | Scientific Literature | |
14. | ALA4005977 | Functional | Cell cycle arrest in human Jurkat T cells assessed as accumulation at G0/G1 phase at 3.2 uM after 24 hrs by propidium iodide staining based flow cytometry (Rvb = 62.7 to 68.6%) | Homo sapiens | 2 | ALA4004831 | cell-based format | Scientific Literature | |
15. | ALA4005978 | Functional | Growth inhibition of human CCRF-CEM cells incubated for 48 hrs by sulforhodamine B assay | Homo sapiens | 10 | ALA4004831 | cell-based format | Scientific Literature | |
16. | ALA4005979 | Functional | Growth inhibition of human HL60 cells incubated for 48 hrs by sulforhodamine B assay | Homo sapiens | 10 | ALA4004831 | cell-based format | Scientific Literature | |
17. | ALA4005980 | Functional | Growth inhibition of human K562 cells incubated for 48 hrs by sulforhodamine B assay | Homo sapiens | 10 | ALA4004831 | cell-based format | Scientific Literature | |
18. | ALA4005981 | Functional | Growth inhibition of human MOLT4 cells incubated for 48 hrs by sulforhodamine B assay | Homo sapiens | 10 | ALA4004831 | cell-based format | Scientific Literature | |
19. | ALA4005982 | Functional | Growth inhibition of human RPMI8226 cells incubated for 48 hrs by sulforhodamine B assay | Homo sapiens | 10 | ALA4004831 | cell-based format | Scientific Literature | |
20. | ALA4005983 | Functional | Growth inhibition of human SR cells incubated for 48 hrs by sulforhodamine B assay | Homo sapiens | 10 | ALA4004831 | cell-based format | Scientific Literature |