#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3889238	B	Radioligand Binding Assay: human or rat P2X7-1321 N1 cells were collected and frozen @−80° C. On the day of the experiment, cell membrane preparations were made according to standard published methods. The total assay volume was 100 μl: 10 μl compound (10×)+(b) 40 μl tracer (2.5×)+50 μl membrane (2×). The tracer used for the assay was tritiated A-804598. The compound can be prepared as described in the literature. (Donnelly-Roberts, D. Neuropharmacology 2009, 56 (1), 223-229.) Compounds, tracer and membranes were incubated for 1 hour @ 4° C. The assay was terminated by filtration (GF/B filters pre-soaked with 0.3% PEI) and washed with washing buffer (Tris-HCl 50 mM).		228		assay format	BindingDB Database
2.	ALA3889240	B	FLIPR Assay: 1321 N1 cells expressing the recombinant human, rat or mouse P2X7 channel was cultured in HyQ DME/(HyClone/Dulbecco's Modified Eagle Medium) high glucose supplemented with 10% Fetal Bovine Serum (FBS) and appropriate selection marker. Cells were seeded at a density of 25000 cells/well (96-well clear bottom black walled plates) in 100 ul volume/well. On the day of the experiment, cell plates were washed with assay buffer, containing (in mM): 130 NaCl, 2 KCl, 1 CaCl2, 1 MgCl2, 10 HEPES, 5 glucose; pH 7.40 and 300 mOs. After the wash, cells were loaded with the Calcium-4 dye (Molecular Device) and incubated in the dark for 60 minutes. Test compounds were prepared at 250ÿ the test concentration in neat DMSO. Intermediate 96-well compound plates were prepared by transferring 1.2 uL of the compound into 300 uL of assay buffer. A further 3x dilution occurred when transferring 50 uL/well of the compound plate to 100 uL/well in the cell plate. Cells were incubate  with test compounds and dye for 30 minutes. Calcium dye fluorescence was monitored in FLIPR as the cells were challenged by adding 50 uL/well of BzATP (final concentration is 250 uM BzATP (human and rat) or 600 (mouse)). The fluorescence change was measured 180 seconds after adding the agonist.		293		cell-based format	BindingDB Database
3.	ALA3889241	B	FLIPR Assay: 1321 N1 cells expressing the recombinant human, rat or mouse P2X7 channel was cultured in HyQ DME/(HyClone/Dulbecco's Modified Eagle Medium) high glucose supplemented with 10% Fetal Bovine Serum (FBS) and appropriate selection marker. Cells were seeded at a density of 25000 cells/well (96-well clear bottom black walled plates) in 100 ul volume/well. On the day of the experiment, cell plates were washed with assay buffer, containing (in mM): 130 NaCl, 2 KCl, 1 CaCl2, 1 MgCl2, 10 HEPES, 5 glucose; pH 7.40 and 300 mOs. After the wash, cells were loaded with the Calcium-4 dye (Molecular Device) and incubated in the dark for 60 minutes. Test compounds were prepared at 250ÿ the test concentration in neat DMSO. Intermediate 96-well compound plates were prepared by transferring 1.2 uL of the compound into 300 uL of assay buffer. A further 3x dilution occurred when transferring 50 uL/well of the compound plate to 100 uL/well in the cell plate. Cells were incubate  with test compounds and dye for 30 minutes. Calcium dye fluorescence was monitored in FLIPR as the cells were challenged by adding 50 uL/well of BzATP (final concentration is 250 uM BzATP (human and rat) or 600 (mouse)). The fluorescence change was measured 180 seconds after adding the agonist.		293		cell-based format	BindingDB Database
4.	ALA4054732	A	Inhibition of CYP3A4 in human liver microsomes using midazolam as substrate after 15 mins by LC-MS/MS analysis	Homo sapiens	5		assay format	Scientific Literature
5.	ALA4054733	A	Inhibition of CYP2D6 in human liver microsomes using dextromethorphan as substrate after 15 mins by LC-MS/MS analysis	Homo sapiens	5		assay format	Scientific Literature
6.	ALA4054734	A	Inhibition of CYP2C9 in human liver microsomes using diclofenac as substrate after 15 mins by LC-MS/MS analysis	Homo sapiens	5		assay format	Scientific Literature
7.	ALA4054735	A	Inhibition of CYP2C19 in human liver microsomes using S-mephenytoin as substrate after 15 mins by LC-MS/MS analysis	Homo sapiens	5		assay format	Scientific Literature
8.	ALA4054736	A	Inhibition of CYP2C8 in human liver microsomes using paclitaxel as substrate after 15 mins by LC-MS/MS analysis	Homo sapiens	5		assay format	Scientific Literature
9.	ALA4054737	A	Inhibition of CYP1A2 in human liver microsomes using phenacetin as substrate after 15 mins by LC-MS/MS analysis	Homo sapiens	5		assay format	Scientific Literature
10.	ALA4054750	P	Solubility of compound in phosphate buffer at pH 7 after 3 days		5		small-molecule physicochemical format	Scientific Literature
11.	ALA4054752	A	Protein binding in rat brain at 5 uM after 5 hrs by equilibrium dialysis method	Rattus norvegicus	5		tissue-based format	Scientific Literature
12.	ALA4054753	P	Solubility of compound in phosphate buffer at pH 2 after 3 days		5		small-molecule physicochemical format	Scientific Literature
13.	ALA4054754	A	Protein binding in human plasma at 1 uM after 6 hrs by equilibrium dialysis method	Homo sapiens	5		cell-free format	Scientific Literature
14.	ALA4054755	A	Protein binding in mouse plasma at 1 uM after 6 hrs by equilibrium dialysis method	Mus musculus	5		cell-free format	Scientific Literature
15.	ALA4054756	A	Protein binding in rat plasma at 1 uM after 6 hrs by equilibrium dialysis method	Rattus norvegicus	5		cell-free format	Scientific Literature
16.	ALA4054757	B	Inhibition of [3H]-dofetilide binding to human ERG expressed in HEK293 cells	Homo sapiens	5		cell-based format	Scientific Literature
17.	ALA4054758	B	Antagonist activity at recombinant rat P2X7 expressed in human 1321N1 cells assessed as inhibition of BzATP-induced calcium flux preincubated for 30 mins followed by BzATP addition measured over 180 secs by FLIPR assay	Rattus norvegicus	5		cell-based format	Scientific Literature
18.	ALA4054759	B	Antagonist activity at recombinant human P2X7 expressed in human 1321N1 cells assessed as inhibition of BzATP-induced calcium flux preincubated for 30 mins followed by BzATP addition measured over 180 secs by FLIPR assay	Homo sapiens	5		cell-based format	Scientific Literature