# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4008166 | B | Inhibition of recombinant N-terminal GST-tagged EED (76 to 441 residues) (unknown origin) expressed in Escherichia coli BL21-CodonPlus(DE3)-RIL assessed as reduction in SAH production using H3[21-44,K27Me0) peptide as substrate measured after 20 mins by LC/MS/MS method | Homo sapiens | 39 | ALA4007397 | assay format | Scientific Literature | |
2. | ALA4008167 | B | Inhibition of EED in human G401 cells assessed as reduction in global H3K27me3 level after 48 hrs by ELISA | Homo sapiens | 5 | ALA4007397 | cell-based format | Scientific Literature | |
3. | ALA4008209 | B | Inhibition of recombinant N-terminal GST-tagged EED (76 to 441 residues) (unknown origin) expressed in Escherichia coli BL21-CodonPlus(DE3)-RIL incubated for 20 mins using EED-H3K27me3 peptide based AlphaScreen competition binding assay | Homo sapiens | 1 | ALA4007397 | assay format | Scientific Literature | |
4. | ALA5127837 | B | Inhibition of biotinylated H3K27Me3 peptide (19 to 33 aa) binding to EED (1 to 441 residues) (unknown origin) expressed in Escherichia coli BL21-Codon plus (DE3)-RIL incubated for 1 hr by alpha screen method | Homo sapiens | 1 | ALA5126528 | assay format | Scientific Literature | |
5. | ALA5127838 | B | Inhibition of EED in human G-401 cells assessed as reduction of global H3K27Me3 level by ELISA | Homo sapiens | 1 | ALA5126528 | cell-based format | Scientific Literature | |
6. | ALA5127839 | B | Inhibition of EED (unknown origin) | Homo sapiens | 15 | ALA5126528 | single protein format | Scientific Literature | |
7. | ALA5237690 | B | Binding affinity to his tagged EED (1 to 441 residues) (unknown origin) incubated for 20 mins in presence of biotinylated H3K27me3 peptide (19 to 33 residues) by alphascreen competitive binding assay | Homo sapiens | 5 | ALA5236564 | single protein format | Scientific Literature |