#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4030854	B	Inhibition of UbcH5c (unknown origin) expressed in human 293T cells assessed as decrease in TNF-alpha-mediated NF-kB activation at 5 uM preincubated for 2 hrs followed by TNF-alpha stimulation for 8 hrs by luciferase reporter gene assay relative to control	Homo sapiens	14	ALA4028851	cell-based format	Scientific Literature
2.	ALA4030855	A	Cytotoxicity against human 293T cells assessed as inhibition of cell viability at 5 uM by CCK-8 assay relative to control	Homo sapiens	14	ALA4028851	cell-based format	Scientific Literature
3.	ALA4030871	B	Inhibition of UbcH5c (unknown origin) at 5 uM preincubated for 15 mins followed by E1, Ub and ATP addition measured after 40 mins by Western blot analysis	Homo sapiens	52	ALA4028851	single protein format	Scientific Literature
4.	ALA4030874	B	Inhibition of UbcH5c in mouse b.End3 cells assessed as decrease in TNF-alpha-mediated IkB-alpha phosphorylation preincubated for 2 hrs followed by TNF-alpha stimulation for 20 mins by Western blot analysis	Mus musculus	6	ALA4028851	cell-based format	Scientific Literature
5.	ALA4030875	B	Inhibition of UbcH5c in mouse b.End3 cells assessed as decrease in TNF-alpha-mediated IkB-alpha degradation preincubated for 2 hrs followed by TNF-alpha stimulation for 20 mins by Western blot analysis	Mus musculus	6	ALA4028851	cell-based format	Scientific Literature
6.	ALA4030876	B	Inhibition of UbcH5c in mouse b.End3 cells assessed as decrease in TNF-alpha-mediated p65 phosphorylation preincubated for 2 hrs followed by TNF-alpha stimulation for 20 mins by Western blot analysis	Mus musculus	6	ALA4028851	cell-based format	Scientific Literature
7.	ALA4030877	B	Inhibition of Ubc13 (unknown origin) at 2.5 to 10 uM preincubated for 20 mins followed by E1, Ub and ATP addition measured after 40 mins iby Western blot analysis	Homo sapiens	6	ALA4028851	single protein format	Scientific Literature
8.	ALA4030878	B	Inhibition of UbcH5c (unknown origin) preincubated for 15 mins followed by E1, Ub and ATP addition measured after 40 mins by Western blot analysis	Homo sapiens	5	ALA4028851	single protein format	Scientific Literature
9.	ALA4030881	B	Binding affinity to human recombinant UbcH5c by SPR analysis	Homo sapiens	5	ALA4028851	single protein format	Scientific Literature
10.	ALA4030896	B	Inhibition of UbcH5c in mouse b.End3 cells assessed as decrease in TNF-alpha-mediated IkB-alpha phosphorylation at 10 uM preincubated for 2 hrs followed by TNF-alpha stimulation for 20 mins by Western blot analysis	Mus musculus	10	ALA4028851	cell-based format	Scientific Literature
11.	ALA4030897	B	Inhibition of UbcH5c in mouse b.End3 cells assessed as decrease in TNF-alpha-mediated IkB-alpha degradation at 10 uM preincubated for 2 hrs followed by TNF-alpha stimulation for 20 mins by Western blot analysis	Mus musculus	10	ALA4028851	cell-based format	Scientific Literature
12.	ALA4030898	B	Binding affinity to human recombinant UbcH5c assessed as association rate constant by SPR analysis	Homo sapiens	4	ALA4028851	single protein format	Scientific Literature
13.	ALA4030899	B	Binding affinity to human recombinant UbcH5c assessed as dissociation rate constant by SPR analysis	Homo sapiens	4	ALA4028851	single protein format	Scientific Literature