#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4041133	Binding	Allosteric inhibition of recombinant human N-terminal GST-tagged WNK1 catalytic domain (1 to 491 residues) expressed in baculovirus expression system using fluorescein-labeled OSR1 peptide as substrate after 3 hrs by HTRF assay	Homo sapiens	12	ALA4038341	assay format	Scientific Literature
2.	ALA4041134	Binding	Allosteric inhibition of WNK1 (unknown origin) expressed in HEK293 cells co-expressing flag-OSR1 assessed as reduction in sorbitol-stimulated OSR1 phosphorylation preincubated with compound for 2.5 hrs followed co-administration with sorbitol measured after 1 hr by AlphaLISA assay	Homo sapiens	3	ALA4038341	cell-based format	Scientific Literature
3.	ALA4041135	Physicochemical	Equilibrium solubility of compound at pH 6.8		3	ALA4038341	small-molecule physicochemical format	Scientific Literature
4.	ALA4041136	ADME	Half life in rat liver microsomes	Rattus norvegicus	3	ALA4038341	assay format	Scientific Literature
5.	ALA4041137	ADME	Clearance in Sprague-Dawley rat at 0.5 mg/kg, iv measured up to 7 hrs post dose by HPLC analysis	Rattus norvegicus	3	ALA4038341	organism-based format	Scientific Literature
6.	ALA4041138	ADME	AUC in Sprague-Dawley rat at 1.5 mg/kg, po measured up to 7 hrs post dose by HPLC analysis	Rattus norvegicus	3	ALA4038341	organism-based format	Scientific Literature
7.	ALA4041139	ADME	Cmax in Sprague-Dawley rat at 1.5 mg/kg, po measured up to 7 hrs post dose by HPLC analysis	Rattus norvegicus	3	ALA4038341	organism-based format	Scientific Literature
8.	ALA4041140	ADME	Oral bioavailability in Sprague-Dawley rat at 1.5 mg/kg measured up to 7 hrs post dose by HPLC analysis	Rattus norvegicus	3	ALA4038341	organism-based format	Scientific Literature
9.	ALA4041146	Functional	Anti-hypertensive activity in po dosed human WNK1 transgenic FVB mouse assessed as reduction in blood pressure administered via gavage measured every 10 minutes for 15 seconds after 6 to 12 hrs	Mus musculus	1	ALA4038341	organism-based format	Scientific Literature
10.	ALA4041147	Unassigned	Ratio of Cp in human WNK1 expressing transgenic FVB mouse at 30 mg/kg, po to IC50 for WNK1 (unknown origin) expressed in HEK293 cells		1	ALA4038341	organism-based format	Scientific Literature
11.	ALA4041148	Functional	Anti-hypertensive activity in human WNK1 transgenic FVB mouse assessed plasma concentration required for SBP reducing activity at 30 mg/kg, po via gavage measured after 7 hrs	Mus musculus	1	ALA4038341	organism-based format	Scientific Literature
12.	ALA4041150	Functional	Anti-hypertensive activity in SHR rat assessed as induction of diuresis administered orally as single dose on day 2,3 and 4 measured for 7 hrs post dose	Rattus norvegicus	1	ALA4038341	assay format	Scientific Literature
13.	ALA4041151	Functional	Anti-hypertensive activity in SRH rat assessed as induction of natriuresis administered orally as single dose on day 2,3 and 4 measured for 7 hrs post dose	Rattus norvegicus	1	ALA4038341	assay format	Scientific Literature
14.	ALA4041152	Functional	Anti-hypertensive activity in SRH rat assessed as induction of kaliuresis administered orally as single dose on day 2,3 and 4 measured for 7 hrs post dose	Rattus norvegicus	1	ALA4038341	assay format	Scientific Literature
15.	ALA4041154	Functional	Anti-hypertensive activity in SRH rat assessed as reduction in blood pressure at 100 mg/kg, po measured for 7 hrs post dose	Rattus norvegicus	1	ALA4038341	organism-based format	Scientific Literature
16.	ALA4041155	Functional	Anti-hypertensive activity in SRH rat assessed as reduction in stroke volume at 100 mg/kg, po measured for 7 hrs post dose	Rattus norvegicus	1	ALA4038341	organism-based format	Scientific Literature
17.	ALA4041156	Functional	Anti-hypertensive activity in SRH rat assessed as reduction in total peripheral resistance at 100 mg/kg, po measured for 7 hrs post dose	Rattus norvegicus	1	ALA4038341	organism-based format	Scientific Literature
18.	ALA4041157	Functional	Increase in heart rate in SRH rat at 100 mg/kg, po measured for 7 hrs post dose	Rattus norvegicus	1	ALA4038341	organism-based format	Scientific Literature
19.	ALA4041158	Functional	Anti-hypertensive activity in po dosed human WNK1 transgenic FVB mouse assessed as reduction in blood pressure administered via gavage measured every 10 minutes for 15 seconds after 2 hrs	Mus musculus	1	ALA4038341	organism-based format	Scientific Literature
20.	ALA4041160	ADME	Plasma concentration in human WNK1 transgenic FVB mouse at 30 mg/kg, po via gavage up to 7 hrs	Mus musculus	1	ALA4038341	organism-based format	Scientific Literature