Drug level in human liver microsomes treated with (R)-2-methyl-N1-((S)-11-oxo-1,2,3,5,10,11-hexahydrobenzo[d]pyrazolo[1,2-a][1,2]diazepin-10-yl)-N4-o-tolylsuccinamide assessed as compound formation at 2 mM preincubated for 3 mins followed by NADPH addition measured after 1 hr in presence of GSH and [13C2-15N-glycine]-GSH by HPLC/MS method
Drug level in rat liver microsomes treated with (R)-2-methyl-N1-((S)-11-oxo-1,2,3,5,10,11-hexahydrobenzo[d]pyrazolo[1,2-a][1,2]diazepin-10-yl)-N4-o-tolylsuccinamide assessed as compound formation at 2 mM preincubated for 3 mins followed by NADPH addition measured after 1 hr in presence of GSH and [13C2-15N-glycine]-GSH by HPLC/MS method
Drug level in mouse liver microsomes treated with (R)-2-methyl-N1-((S)-11-oxo-1,2,3,5,10,11-hexahydrobenzo[d]pyrazolo[1,2-a][1,2]diazepin-10-yl)-N4-o-tolylsuccinamide assessed as compound formation at 2 mM preincubated for 3 mins followed by NADPH addition measured after 1 hr in presence of GSH and [13C2-15N-glycine]-GSH by HPLC/MS method