# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4018226 | F | Osteogenic activity in mouse MC3T3-E1 cells assessed as increase in mineralization at 30 uM measured after 10 days in presence of BMP2 inhibitor noggin by alizarin red S staining based assay | Mus musculus | 2 | ALA4017476 | cell-based format | Scientific Literature | |
2. | ALA4018227 | F | Osteogenic activity in mouse MC3T3-E1 cells assessed as increase in mineralization at 30 uM measured after 10 days in presence of type-1 BMP receptor kinase inhibitor LDN-193189 by alizarin red S staining based assay | Mus musculus | 2 | ALA4017476 | cell-based format | Scientific Literature | |
3. | ALA4018228 | F | Inhibition of RANKL-stimulated osteoclastogenesis in mouse RAW264 cells assessed as reduction in TRAP activity at 20 uM measured after 72 hrs by microscopic analysis | Mus musculus | 4 | ALA4017476 | cell-based format | Scientific Literature | |
4. | ALA4018231 | F | Osteogenic activity in mouse MC3T3-E1 cells assessed as increase in ALP activity at 30 uM measured after 4 days relative to control | Mus musculus | 17 | ALA4017476 | cell-based format | Scientific Literature | |
5. | ALA4018232 | F | Osteogenic activity in mouse MC3T3-E1 cells assessed as increase in mineralization at 30 uM measured after 10 days by alizarin red S staining based assay | Mus musculus | 20 | ALA4017476 | cell-based format | Scientific Literature | |
6. | ALA4018236 | A | Cytotoxicity against mouse MC3T3-E1 cells assessed as cell number at 30 uM measured after 4 days by WST-8 assay relative to control | Mus musculus | 22 | ALA4017476 | cell-based format | Scientific Literature | |
7. | ALA4018238 | B | Activation of BMP signaling pathway in mouse MC3T3-E1 cells assessed as increase in Runx2 mRNA expression after 2 days by SYBR green-dye based RT-PCR method | Mus musculus | 2 | ALA4017476 | cell-based format | Scientific Literature | |
8. | ALA4018239 | B | Activation of BMP signaling pathway in mouse MC3T3-E1 cells assessed as increase in Osterix mRNA expression after 2 days by SYBR green-dye based RT-PCR method | Mus musculus | 2 | ALA4017476 | cell-based format | Scientific Literature | |
9. | ALA4018240 | B | Activation of BMP signaling pathway in mouse MC3T3-E1 cells assessed as increase in Alpl mRNA expression after 2 days by SYBR green-dye based RT-PCR method | Mus musculus | 2 | ALA4017476 | cell-based format | Scientific Literature | |
10. | ALA4018241 | B | Activation of BMP signaling pathway in mouse MC3T3-E1 cells assessed as increase in Bglap mRNA expression after 2 days by SYBR green-dye based RT-PCR method | Mus musculus | 2 | ALA4017476 | cell-based format | Scientific Literature | |
11. | ALA4018242 | B | Activation of BMP signaling pathway in mouse MC3T3-E1 cells assessed as increase in Runx2 mRNA expression at 10 uM after 2 days by SYBR green-dye based RT-PCR method relative to control | Mus musculus | 2 | ALA4017476 | cell-based format | Scientific Literature | |
12. | ALA4018243 | B | Activation of BMP signaling pathway in mouse MC3T3-E1 cells assessed as increase in Osterix mRNA expression at 10 uM after 2 days by SYBR green-dye based RT-PCR method relative to control | Mus musculus | 2 | ALA4017476 | cell-based format | Scientific Literature | |
13. | ALA4018244 | B | Activation of BMP signaling pathway in mouse MC3T3-E1 cells assessed as increase in Alpl mRNA expression at 10 uM after 2 days by SYBR green-dye based RT-PCR method relative to control | Mus musculus | 2 | ALA4017476 | cell-based format | Scientific Literature | |
14. | ALA4018245 | B | Activation of BMP signaling pathway in mouse MC3T3-E1 cells assessed as increase in Bglap mRNA expression at 10 uM after 2 days by SYBR green-dye based RT-PCR method relative to control | Mus musculus | 2 | ALA4017476 | cell-based format | Scientific Literature | |
15. | ALA4018246 | B | Induction of p38 MAPK phosphorylation in mouse MC3T3-E1 cells assessed as ratio of phosphorylated p38 to unphosphorylated p38 protein level at 30 uM measured after 5 mins by Western blot analysis relative to control | Mus musculus | 2 | ALA4017476 | cell-based format | Scientific Literature | |
16. | ALA4018247 | B | Induction of Smad1/5/8 phosphorylation in mouse MC3T3-E1 cells assessed as ratio of phosphorylated Smad1/5/8 to actin beta protein level at 30 uM measured after 5 mins by Western blot analysis relative to control | Mus musculus | 2 | ALA4017476 | cell-based format | Scientific Literature | |
17. | ALA4018248 | F | Osteogenic activity in mouse MC3T3-E1 cells assessed as increase in mineralization at 30 uM measured after 10 days in presence of p38 MAPK inhibitor SB203580 by alizarin red S staining based assay | Mus musculus | 2 | ALA4017476 | cell-based format | Scientific Literature | |
18. | ALA4018249 | F | Inhibition of RANKL-stimulated osteoclastogenesis in mouse RAW264 cells assessed as reduction in TRAP-positive multinucleated cells at 20 uM measured after 72 hrs by TRAP staining based microscopic analysis | Mus musculus | 2 | ALA4017476 | cell-based format | Scientific Literature | |
19. | ALA4018250 | B | Induction of p38 MAPK phosphorylation in mouse MC3T3-E1 cells assessed as ratio of phosphorylated p38 to unphosphorylated p38 protein level at 30 uM measured after 15 mins by Western blot analysis relative to control | Mus musculus | 2 | ALA4017476 | cell-based format | Scientific Literature | |
20. | ALA4018251 | B | Induction of p38 MAPK phosphorylation in mouse MC3T3-E1 cells assessed as ratio of phosphorylated p38 to unphosphorylated p38 protein level at 30 uM measured after 30 mins by Western blot analysis relative to control | Mus musculus | 2 | ALA4017476 | cell-based format | Scientific Literature |