#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4018226	F	Osteogenic activity in mouse MC3T3-E1 cells assessed as increase in mineralization at 30 uM measured after 10 days in presence of BMP2 inhibitor noggin by alizarin red S staining based assay	Mus musculus	2		cell-based format	Scientific Literature
2.	ALA4018227	F	Osteogenic activity in mouse MC3T3-E1 cells assessed as increase in mineralization at 30 uM measured after 10 days in presence of type-1 BMP receptor kinase inhibitor LDN-193189 by alizarin red S staining based assay	Mus musculus	2		cell-based format	Scientific Literature
3.	ALA4018228	F	Inhibition of RANKL-stimulated osteoclastogenesis in mouse RAW264 cells assessed as reduction in TRAP activity at 20 uM measured after 72 hrs by microscopic analysis	Mus musculus	4		cell-based format	Scientific Literature
4.	ALA4018231	F	Osteogenic activity in mouse MC3T3-E1 cells assessed as increase in ALP activity at 30 uM measured after 4 days relative to control	Mus musculus	17		cell-based format	Scientific Literature
5.	ALA4018232	F	Osteogenic activity in mouse MC3T3-E1 cells assessed as increase in mineralization at 30 uM measured after 10 days by alizarin red S staining based assay	Mus musculus	20		cell-based format	Scientific Literature
6.	ALA4018233	F	Osteogenic activity in mouse MC3T3-E1 cells assessed as increase in ALP activity at 10 uM measured after 4 days relative to control	Mus musculus	2		cell-based format	Scientific Literature
7.	ALA4018234	F	Osteogenic activity in mouse MC3T3-E1 cells assessed as increase in mineralization at 10 uM measured after 10 days by alizarin red S staining based assay	Mus musculus	2		cell-based format	Scientific Literature
8.	ALA4018236	A	Cytotoxicity against mouse MC3T3-E1 cells assessed as cell number at 30 uM measured after 4 days by WST-8 assay relative to control	Mus musculus	22		cell-based format	Scientific Literature
9.	ALA4018238	B	Activation of BMP signaling pathway in mouse MC3T3-E1 cells assessed as increase in Runx2 mRNA expression after 2 days by SYBR green-dye based RT-PCR method	Mus musculus	2		cell-based format	Scientific Literature
10.	ALA4018239	B	Activation of BMP signaling pathway in mouse MC3T3-E1 cells assessed as increase in Osterix mRNA expression after 2 days by SYBR green-dye based RT-PCR method	Mus musculus	2		cell-based format	Scientific Literature
11.	ALA4018240	B	Activation of BMP signaling pathway in mouse MC3T3-E1 cells assessed as increase in Alpl mRNA expression after 2 days by SYBR green-dye based RT-PCR method	Mus musculus	2		cell-based format	Scientific Literature
12.	ALA4018241	B	Activation of BMP signaling pathway in mouse MC3T3-E1 cells assessed as increase in Bglap mRNA expression after 2 days by SYBR green-dye based RT-PCR method	Mus musculus	2		cell-based format	Scientific Literature
13.	ALA4018242	B	Activation of BMP signaling pathway in mouse MC3T3-E1 cells assessed as increase in Runx2 mRNA expression at 10 uM after 2 days by SYBR green-dye based RT-PCR method relative to control	Mus musculus	2		cell-based format	Scientific Literature
14.	ALA4018243	B	Activation of BMP signaling pathway in mouse MC3T3-E1 cells assessed as increase in Osterix mRNA expression at 10 uM after 2 days by SYBR green-dye based RT-PCR method relative to control	Mus musculus	2		cell-based format	Scientific Literature
15.	ALA4018244	B	Activation of BMP signaling pathway in mouse MC3T3-E1 cells assessed as increase in Alpl mRNA expression at 10 uM after 2 days by SYBR green-dye based RT-PCR method relative to control	Mus musculus	2		cell-based format	Scientific Literature
16.	ALA4018245	B	Activation of BMP signaling pathway in mouse MC3T3-E1 cells assessed as increase in Bglap mRNA expression at 10 uM after 2 days by SYBR green-dye based RT-PCR method relative to control	Mus musculus	2		cell-based format	Scientific Literature
17.	ALA4018246	B	Induction of p38 MAPK phosphorylation in mouse MC3T3-E1 cells assessed as ratio of phosphorylated p38 to unphosphorylated p38 protein level at 30 uM measured after 5 mins by Western blot analysis relative to control	Mus musculus	2		cell-based format	Scientific Literature
18.	ALA4018247	B	Induction of Smad1/5/8 phosphorylation in mouse MC3T3-E1 cells assessed as ratio of phosphorylated Smad1/5/8 to actin beta protein level at 30 uM measured after 5 mins by Western blot analysis relative to control	Mus musculus	2		cell-based format	Scientific Literature
19.	ALA4018248	F	Osteogenic activity in mouse MC3T3-E1 cells assessed as increase in mineralization at 30 uM measured after 10 days in presence of p38 MAPK inhibitor SB203580 by alizarin red S staining based assay	Mus musculus	2		cell-based format	Scientific Literature
20.	ALA4018249	F	Inhibition of RANKL-stimulated osteoclastogenesis in mouse RAW264 cells assessed as reduction in TRAP-positive multinucleated cells at 20 uM measured after 72 hrs by TRAP staining based microscopic analysis	Mus musculus	2		cell-based format	Scientific Literature