#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4024541	A	Half life in mouse liver microsomes at 0.8 uM by LC-MS/MS analysis	Mus musculus	48		assay format	Scientific Literature
2.	ALA4024542	A	Intrinsic clearance in mouse liver microsomes at 0.8 uM by LC-MS/MS analysis	Mus musculus	48		assay format	Scientific Literature
3.	ALA4222544	B	Binding affinity biotinylated human GST-tagged DCN1 assessed as inhibition of protein interaction with AlexaFluor 488-tagged and N-terminally acetylated human UBE2M (1 to 12 residues) after 1 hr by TR-FRET assay	Homo sapiens	62		single protein format	Scientific Literature
4.	ALA4222545	P	Solubility of the compound at pH 7.4 after 18 hrs by UV-spectroscopy		61		small-molecule physicochemical format	Scientific Literature
5.	ALA4222546	A	Permeability of the compound at 10 uM by PAMPA		61		assay format	Scientific Literature
6.	ALA4222547	B	Binding affinity biotinylated human GST-tagged DCN1 by ITC method	Homo sapiens	10		single protein format	Scientific Literature
7.	ALA4222553	B	Inhibition of human DCN1-mediated cullin neddylation assessed as reduction in transfer of fluorescein-5-maleimide-tagged NEDD8 from N-terminally acetylated human UBE2M to CUL2-CTD measured for 10 sec by pulse-chase assay	Homo sapiens	10		single protein format	Scientific Literature
8.	ALA4222554	A	Growth inhibition of human BJ cells	Homo sapiens	10		cell-based format	Scientific Literature
9.	ALA4222555	F	Growth inhibition of human HCC95 cells	Homo sapiens	10		cell-based format	Scientific Literature
10.	ALA4222556	B	Binding affinity to DCN1 in human HCC95 cells assessed as enhancement of thermal stability at 10 uM pre-incubated for 1 hr followed by heating at 39 to 60 degC for 3 mins by cellular thermal shift assay	Homo sapiens	4		cell-based format	Scientific Literature
11.	ALA4222557	B	Inhibition of DCN1-mediated cullin neddylation in human HCC95 cells assessed as reduction in transfer of NEDD8 to CUL1 at 20 uM after 24 hrs by Western blot analysis	Homo sapiens	2		cell-based format	Scientific Literature
12.	ALA4222558	B	Inhibition of human NAE1-UBA3 E1 activating enzyme assessed as reduction in CUL3 neddylation by measuring inhibition of UBE2M thiol-ester formation at 50 uM up to 60 mins by pulse-chase assay	Homo sapiens	2		protein complex format	Scientific Literature
13.	ALA4222559	B	Inhibition of human DCN3 stimulated N-terminal cys-tagged NEDD8 transfer from UBE2F to CUL2(CTD)-RBX1 at 10 uM measured up to 2 mins by pulse-chase assay	Homo sapiens	2		single protein format	Scientific Literature
14.	ALA4222560	B	Inhibition of human RBX1-catalyzed CUL2(CTD) neddylation assessed as reduction in transfer of N-terminal cys-tagged NEDD8 from UBE2M to CUL2-CTD at 12.5 uM measured up to 6 mins by pulse-chase assay	Homo sapiens	4		assay format	Scientific Literature
15.	ALA4222561	B	Inhibition of DCN1-mediated cullin neddylation in human HCC95 cells assessed as reduction in transfer of NEDD8 to CUL2 at 20 uM after 24 hrs by Western blot analysis	Homo sapiens	2		cell-based format	Scientific Literature
16.	ALA4222562	B	Inhibition of DCN1-mediated cullin neddylation in human HCC95 cells assessed as reduction in transfer of NEDD8 to CUL3 at 20 uM after 24 hrs by Western blot analysis	Homo sapiens	2		cell-based format	Scientific Literature
17.	ALA4222563	B	Inhibition of DCN1-mediated cullin neddylation in human HCC95 cells assessed as reduction in transfer of NEDD8 to CUL4A at 20 uM after 24 hrs by Western blot analysis	Homo sapiens	2		cell-based format	Scientific Literature
18.	ALA4222564	B	Inhibition of DCN1-mediated cullin neddylation in human HCC95 cells assessed as reduction in transfer of NEDD8 to CUL5 at 20 uM after 24 hrs by Western blot analysis	Homo sapiens	2		cell-based format	Scientific Literature
19.	ALA4222566	B	Inhibition of human DCN1 stimulated N-terminal cys-tagged NEDD8 transfer from UBE2F to CUL2(CTD)-RBX1 at 10 uM measured up to 2 mins by pulse-chase assay	Homo sapiens	2		single protein format	Scientific Literature