Inhibition of Cav3.2 alpha1H (unknown origin) expressed in HEK293 cells assessed as reduction in KCl-induced depolarization mediated intracellular Ca2+ level at 10 uM by fluo3/AM based high throughput screening assay relative to control
Inhibition of recombinant human CYP1A2 expressed in baculosomes assessed as residual activity at 10 uM using vivid EOMCC as substrate after 15 mins in presence of NADPH by fluorescence assay relative to control
Inhibition of recombinant human CYP2D6 expressed in baculosomes assessed as residual activity at 10 uM using vivid EOMCC/MOBFC as substrate after 15 mins in presence of NADPH by fluorescence assay relative to control
Inhibition of recombinant human CYP2C9 expressed in baculosomes assessed as residual activity at 10 uM using vivid BOMCC/BOMF/OOMR as substrate after 15 mins in presence of NADPH by fluorescence assay relative to control
Inhibition of recombinant human CYP3A4 expressed in baculosomes assessed as residual activity at 10 uM using vivid BOMCC/DBOMF/BOMR as substrate after 15 mins in presence of NADPH by fluorescence assay relative to control