# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4015725 | B | Binding affinity to CD14/MD2 (unknown origin) expressed in HEK-Blue cells co-expressing TLR4 assessed as inhibition of LPS-induced TLR4 signaling preincubated for 30 mins followed by LPS stimulation measured after overnight incubation by secreted embryonic alkaline phosphatase reporter gene assay | Homo sapiens | 6 | cell-based format | Scientific Literature | ||
2. | ALA4015726 | A | Activation of TLR4 signaling (unknown origin) expressed in HEK-Blue cells after 30 mins by secreted embryonic alkaline phosphatase reporter gene assay | Homo sapiens | 9 | cell-based format | Scientific Literature | ||
3. | ALA4015728 | B | Binding affinity to CD14/MD2 (unknown origin) expressed in HEK-Blue cells co-expressing TLR4 assessed as inhibition of PHA-P-induced TLR4 signaling preincubated for 30 mins followed by PHA-P stimulation measured after overnight incubation by secreted embryonic alkaline phosphatase reporter gene assay | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
4. | ALA4015729 | B | Binding affinity to CD14/MD2 in human PBMC assessed as inhibition of LPS-induced TLR4 signaling by measuring decrease in TNFalpha secretion at 1 to 10 uM preincubated for 30 mins followed by LPS stimulation for 18 hrs by ELISA | Homo sapiens | 2 | protein complex format | Scientific Literature | ||
5. | ALA4015730 | B | Binding affinity to CD14/MD2 in human PBMC assessed as inhibition of LPS-induced TLR4 signaling by measuring decrease in IL-6 secretion at 1 to 10 uM preincubated for 30 mins followed by LPS stimulation for 18 hrs by ELISA | Homo sapiens | 2 | protein complex format | Scientific Literature | ||
6. | ALA4015731 | B | Binding affinity to CD14/MD2 in human PBMC assessed as inhibition of LPS-induced TLR4 signaling by measuring decrease in IL-8 secretion at 1 to 10 uM preincubated for 30 mins followed by LPS stimulation for 18 hrs by ELISA | Homo sapiens | 2 | protein complex format | Scientific Literature | ||
7. | ALA4015732 | B | Binding affinity to CD14/MD2 in mouse RAW-Blue cells assessed as inhibition of LPS-induced TLR4 signaling preincubated for 30 mins followed by LPS stimulation measured after overnight incubation by secreted embryonic alkaline phosphatase reporter gene assay | Mus musculus | 2 | cell-based format | Scientific Literature | ||
8. | ALA4015733 | B | Binding affinity to CD14/MD2 in Balb/c mouse splenocytes assessed as inhibition of LPS-induced TLR4-MyD88 pathway by measuring reduction in TNFalpha expression at 1 to 10 uM preincubated for 30 mins followed by LPS stimulation for 5 hrs by qRT-PCR method | Mus musculus | 2 | cell-based format | Scientific Literature | ||
9. | ALA4015734 | B | Binding affinity to CD14/MD2 (unknown origin) expressed in HEK-Blue cells co-expressing TLR4 assessed as inhibition of LPS-induced TLR4 signaling by measuring reduction in IL-8 secretion preincubated for 30 mins followed by LPS stimulation measured after overnight incubation by ELISA | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
10. | ALA4015735 | B | Binding affinity to CD14/MD2 (unknown origin) expressed in HEK-Blue cells co-expressing TLR4 assessed as inhibition of PHA-P-induced TLR4 signaling by measuring reduction in IL-8 secretion preincubated for 30 mins followed by PHA-P stimulation measured after overnight incubation by ELISA | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
11. | ALA4015736 | A | Cytotoxicity against HEK-Blue cells assessed as reduction in cell viability up to 10 uM measured after overnight incubation by MTT assay | Homo sapiens | 2 | cell-based format | Scientific Literature |