# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4122262 | B | Inhibition of recombinant C-terminal His10-tagged human Hepsin (R45 to L17 residues) D161E/ R162K double mutant expressed in mouse NS0 cells using Boc-QRR-AMC as substrate after 15 mins by automated fluorescence assay | Homo sapiens | 35 | cell-based format | Scientific Literature | ||
2. | ALA4122263 | B | Inhibition of recombinant C-terminal His10-tagged human uPA (M1 to L431 residues) expressed in mouse NS0 cells using Z-GGR-AMC as substrate after 15 mins by automated fluorescence assay | Homo sapiens | 34 | cell-based format | Scientific Literature | ||
3. | ALA4122270 | B | Inhibition of doxycycline induced Hepsin in human MCF10A cells using Boc-QRR-AMC as substrate preincubated for 24 hrs followed by doxycycline induction for 24 hrs and subsequent addition of substrate by fluorescence assay | Homo sapiens | 5 | cell-based format | Scientific Literature | ||
4. | ALA4122271 | B | Inhibition of doxycycline induced Hepsin in human MCF10A cells assessed as prevention in loss of HAI-1 level at 10 uM preincubated for 30 mins followed by doxycycline addition measured after 6 hrs by Immunoblot assay | Homo sapiens | 5 | cell-based format | Scientific Literature | ||
5. | ALA4122274 | B | Inhibition of hepsin-mediated conversion of Pro-HGF into active form in human Hs578T cells assessed as decrease in MET phosphorylation at 1 to 10 uM preincubated for 30 mins followed by recombinant human pro-HGF addition measured after 30 mins by immunoblot method | Homo sapiens | 5 | cell-based format | Scientific Literature | ||
6. | ALA4122281 | B | Inhibition of doxycycline induced hepsin-mediated loss of desmosomal junctions in human MCF10A cells at 1 to 10 uM preincubated for 30 mins followed by doxycycline addition measured after 6 hrs by desmoglein 2 staining-based confocal immunofluorescence microscopic analysis | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
7. | ALA4122307 | B | Inhibition of recombinant Hepsin (unknown origin) | Homo sapiens | 1 | single protein format | Scientific Literature |