| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4122971 | B | Displacement of C-terminally biotinylated-H3K4me3 (1 to 21 residues) peptide from recombinant His6-tagged human KDM5A (PHD3) (1542 to 1660 residues) expressed in Escherichia coli 2(DE3)pLysS Rosetta competent cells preincubated for 15 mins followed by peptide addition measured after 1 hr by luminescence-based AlphaScreen assay | Homo sapiens | 18 | ALA4118181 | cell-based format | Scientific Literature | |
| 2. | ALA4122972 | B | Inhibition of KDM5A (M1 to L801 residues) PHD2/3 deletion mutant (unknown origin) demethylation activity preincubated for 10 mins followed by peptide addition measured after 3 mins by MALDI-TOF-MS analysis | Homo sapiens | 5 | ALA4118181 | single protein format | Scientific Literature | |
| 3. | ALA4122973 | B | Mixed type inhibition of KDM5A (L88 to G353 residues) ARID/PHD1/2/3 deletion mutant (unknown origin) demethylation activity using H3(1-21)K4me3 peptide as substrate measured every 30 seconds over 25 mins by formaldehyde dehydrogenase coupled activity assay based Michaelis-Menten kinetic plot analysis | Homo sapiens | 1 | ALA4118181 | single protein format | Scientific Literature | |
| 4. | ALA4122974 | B | Inhibition of KDM5A (L88 to G353 residues) ARID/PHD1/2/3 deletion mutant (unknown origin) demethylation activity preincubated for 10 mins followed by peptide addition measured after 4 mins by MALDI-TOF-MS analysis | Homo sapiens | 5 | ALA4118181 | single protein format | Scientific Literature | |
| 5. | ALA4122975 | B | Displacement of C-terminally biotinylated-H3K4me3 (1 to 21 residues) peptide from KDM7A (PHD) (unknown origin) preincubated for 15 mins followed by peptide addition measured after 1 hr by luminescence-based AlphaScreen assay | Homo sapiens | 15 | ALA4118181 | single protein format | Scientific Literature | |
| 6. | ALA4122976 | B | Displacement of C-terminally biotinylated-H3K4me3 (1 to 21 residues) peptide from KDM7B (PHD) (unknown origin) preincubated for 15 mins followed by peptide addition measured after 1 hr by luminescence-based AlphaScreen assay | Homo sapiens | 15 | ALA4118181 | single protein format | Scientific Literature | |
| 7. | ALA4122977 | B | Displacement of C-terminally biotinylated-H3K4me3 (1 to 21 residues) peptide from KDM7C (PHD) (unknown origin) preincubated for 15 mins followed by peptide addition measured after 1 hr by luminescence-based AlphaScreen assay | Homo sapiens | 15 | ALA4118181 | single protein format | Scientific Literature | |
| 8. | ALA4122978 | B | Binding affinity to biotin-His6 peptide (unknown origin) by counterscreen assay | Not specified | 12 | ALA4118181 | assay format | Scientific Literature | |
| 9. | ALA4122979 | B | Displacement of C-terminally biotinylated-H3K4me3 (1 to 21 residues) peptide from KDM7A (PHD-JmjC) (unknown origin) preincubated for 15 mins followed by peptide addition measured after 1 hr by luminescence-based AlphaScreen assay | Homo sapiens | 6 | ALA4118181 | single protein format | Scientific Literature | |
| 10. | ALA4122981 | B | Inhibition of KDM7A (PHD-JmjC) (unknown origin) demethylation activity at 100 uM preincubated for 10 mins followed by H3(1-15)K9me2 peptide substrate addition by MALDI-TOF-MS analysis | Homo sapiens | 7 | ALA4118181 | single protein format | Scientific Literature | |
| 11. | ALA4122982 | B | Displacement of C-terminally biotinylated-H3K4me3 (1 to 21 residues) peptide from KDM7B (PHD-JmjC) (unknown origin) preincubated for 15 mins followed by peptide addition measured after 1 hr by luminescence-based AlphaScreen assay | Homo sapiens | 3 | ALA4118181 | single protein format | Scientific Literature | |
| 12. | ALA4122984 | B | Inhibition of KDM7B (PHD-JmjC) (unknown origin) demethylation activity at 100 uM preincubated for 10 mins followed by H3(1-15)K4me3K9me2 peptide substrate addition by MALDI-TOF-MS analysis | Homo sapiens | 7 | ALA4118181 | single protein format | Scientific Literature | |
| 13. | ALA4122985 | B | Mixed type inhibition of KDM5A (L88 to G353 residues) ARID/PHD1/2/3 deletion mutant (unknown origin) demethylation activity using H3(1-21)K4me3 peptide as substrate measured every 30 seconds over 25 mins by formaldehyde dehydrogenase coupled activity assay based Lineweaver-Burk plot analysis | Homo sapiens | 1 | ALA4118181 | single protein format | Scientific Literature | |
| 14. | ALA4122986 | B | Substrate activity at KDM5A (L88 to G353 residues) ARID/PHD1/2/3 deletion mutant (unknown origin) demethylation activity using H3(1-21)K4me3 peptide as substrate measured every 30 seconds over 25 mins in absence of formaldehyde | Homo sapiens | 1 | ALA4118181 | single protein format | Scientific Literature |
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