# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4136597 | B | Inhibition of recombinant human His6-tagged USP28-5 (1 to 1077 residues) using Ub-Rh110 as substrate pretreated for 30 mins followed by substrate addition measured after 20 mins by fluorescence assay | Homo sapiens | 8 | ALA4130588 | assay format | Scientific Literature | |
2. | ALA4136598 | B | Inhibition of recombinant human His6-tagged USP25 expressed in baculovirus infected Sf21 insect cells using Ub-Rh110 as substrate pretreated for 30 mins followed by substrate addition measured after 45 mins by fluorescence assay | Homo sapiens | 8 | ALA4130588 | cell-based format | Scientific Literature | |
3. | ALA4418451 | B | Inhibition of TEV-cleavable hexa-histidine tagged USP28 (1 to 1077 residues) (unknown origin) using ubiquitin-rhodamine 110 as substrate preincubated for 30 mins followed by substrate addition and measured after 20 mins by fluorescence based assay | Homo sapiens | 21 | ALA4418307 | single protein format | Patent Bioactivity Data | |
4. | ALA4418452 | B | Inhibition of recombinant C-terminal 6-His tagged human USP25 using ubiquitin-rhodamine 110 as substrate preincubated for 30 mins followed by substrate addition and measured after 45 mins by fluorescence based assay | Homo sapiens | 21 | ALA4418307 | single protein format | Patent Bioactivity Data |