# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4155117 | B | Inhibition of Escherichia coli C-terminal His6-tagged DNA gyrase B expressed in Escherichia coli BL21(DE3) preincubated for 10 mins followed by ATP addition measured after 30 mins by purine nucleoside phosphorylase enzyme coupled spectrophotometric method | Escherichia coli | 16 | ALA4152269 | assay format | Scientific Literature | |
2. | ALA4155118 | B | Inhibition of Escherichia coli C-terminal His6-tagged topoisomerase-4 expressed in Escherichia coli BL21(DE3) preincubated for 10 mins followed by ATP addition measured after 30 mins by purine nucleoside phosphorylase enzyme coupled spectrophotometric method | Escherichia coli | 7 | ALA4152269 | assay format | Scientific Literature | |
3. | ALA4155124 | B | Inhibition of Escherichia coli C-terminal His6-tagged DNA gyrase B expressed in Escherichia coli BL21(DE3) assessed as residual activity at 1 uM preincubated for 10 mins followed by ATP addition measured after 30 mins by purine nucleoside phosphorylase enzyme coupled spectrophotometric method relative to control | Escherichia coli | 39 | ALA4152269 | assay format | Scientific Literature | |
4. | ALA4155126 | B | Inhibition of Staphylococcus aureus DNA gyrase B preincubated for 10 mins followed by ATP addition measured after 30 mins by purine nucleoside phosphorylase enzyme coupled spectrophotometric method | Staphylococcus aureus | 11 | ALA4152269 | single protein format | Scientific Literature | |
5. | ALA4155130 | B | Inhibition of Staphylococcus aureus topoisomerase-4 assessed as residual activity preincubated for 10 mins followed by ATP addition measured after 30 mins by purine nucleoside phosphorylase enzyme coupled spectrophotometric method | Staphylococcus aureus | 9 | ALA4152269 | assay format | Scientific Literature | |
6. | ALA4155131 | F | Growth inhibition of Staphylococcus aureus ATCC 25923 at 50 uM after 24 hrs by broth microdilution method relative to control | Staphylococcus aureus | 28 | ALA4152269 | organism-based format | Scientific Literature | |
7. | ALA4155132 | F | Growth inhibition of Enterococcus faecalis ATCC 29212 at 50 uM after 24 hrs by broth microdilution method relative to control | Enterococcus faecalis | 28 | ALA4152269 | organism-based format | Scientific Literature | |
8. | ALA4155133 | F | Growth inhibition of Escherichia coli ATCC 25922 at 50 uM after 24 hrs by broth microdilution method relative to control | Escherichia coli | 28 | ALA4152269 | organism-based format | Scientific Literature | |
9. | ALA4155134 | F | Growth inhibition of Pseudomonas aeruginosa ATCC 27853 at 50 uM after 24 hrs by broth microdilution method relative to control | Pseudomonas aeruginosa | 28 | ALA4152269 | organism-based format | Scientific Literature | |
10. | ALA4155135 | F | Growth inhibition of tolC deficient Escherichia coli JW5503 at 50 uM after 24 hrs by broth microdilution method relative to control | Escherichia coli | 28 | ALA4152269 | organism-based format | Scientific Literature | |
11. | ALA4155136 | F | Growth inhibition of lpxC deficient Escherichia coli JD17464 at 50 uM after 24 hrs by broth microdilution method relative to control | Escherichia coli | 28 | ALA4152269 | organism-based format | Scientific Literature |