| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4139276 | B | Inhibition of human SMS2 expressed in HEK293T cells using C2-ceramide as substrate preincubated for 60 mins followed by substrate addition measured after 30 mins by RapidFire/mass spectrometry assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 2. | ALA4139283 | A | Cytotoxicity against HEK293T cells by CellTiter-Glo luminescent cell viability assay relative to control | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 3. | ALA4139288 | B | Inhibition of full length C-terminal FLAG tagged human SMS2 expressed in HEK293 cell membranes using DMPC-d72 and C17-ceramide as substrate after 15 mins by RapidFire/mass spectrometry assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 4. | ALA4139289 | B | Inhibition of full length C-terminal FLAG tagged human SMS2 expressed in HEK293 cell membranes using DMPC-d72 and C17-ceramide as substrate preincubated for 60 mins followed by substrate addition measured after 15 mins by RapidFire/mass spectrometry assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 5. | ALA4139290 | B | Reversible inhibition of full length C-terminal FLAG tagged human SMS2 expressed in HEK293 cells at 3 uM using DMPC-d72 and C17-ceramide as substrate preincubated for 60 mins followed by 33 fold diluted substrate addition measured after 30 mins by RapidFire/mass spectrometry assay relative to control | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 6. | ALA4139291 | A | Inhibition of human SMS1 | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 7. | ALA4139292 | B | Inhibition of human SMS2 | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 8. | ALA4139293 | B | Selectivity index, ratio of IC50 for human SMS1 to IC50 for human SMS2 | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 9. | ALA4139294 | B | Inhibition of full length C-terminal FLAG tagged human SMS2 expressed in HEK293 cell membranes at 3 uM using DMPC-d72 and C17-ceramide as substrate preincubated for 60 mins followed by substrate addition measured after 30 mins by RapidFire/mass spectrometry assay | Homo sapiens | 3 | cell-based format | Scientific Literature | ||
| 10. | ALA4139295 | B | Time-dependent inhibition of full length C-terminal FLAG tagged human SMS2 expressed in HEK293 cell membranes using DMPC-d72 and C17-ceramide as substrate preincubated with enzyme followed by substrate addition by RapidFire/mass spectrometry assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 11. | ALA4628266 | B | Inhibition of human recombinant C-terminal FLAG-tagged SMS2 expressed in Freestyle293 cell membrane using C14-phosphatidylcholineD72 and C17-ceramide pre-incubated for 60 mins followed by incubation with substrate for 30 mins measured by RapidFire/MS assay | Homo sapiens | 17 | cell-based format | Scientific Literature | ||
| 12. | ALA4628267 | B | Inhibition of mouse recombinant C-terminal FLAG-tagged SMS2 expressed in mammalian expression system using C14-phosphatidylcholineD72 and C17-ceramide pre-incubated for 60 mins followed by incubation with substrate for 30 mins measured by RapidFire/MS assay | Mus musculus | 17 | assay format | Scientific Literature | ||
| 13. | ALA4628268 | A | Inhibition of human recombinant C-terminal FLAG-tagged SMS1 expressed in mammalian expression system using C14-phosphatidylcholineD72 and C17-ceramide pre-incubated for 60 mins followed by incubation with substrate for 30 mins measured by RapidFire/MS assay | Homo sapiens | 17 | assay format | Scientific Literature |
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