| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4140999 | B | Inhibition of secreted cathepsin D in human MDA-MB-231 cells using Mca-Gly-Lys-Pro-Ile-Leu-PhePhe-Arg-Leu-Lys-(Dnp)-D-Arg-NH2 as substrate pretreated for 3 days under pH 6.6 conditioned media followed by substrate addition measured at 5 mins interval for 2 hrs by fluorescence assay | Homo sapiens | 3 | ALA4138143 | cell-based format | Scientific Literature | |
| 2. | ALA4141000 | B | Inhibition of human liver cathepsin D using Mca-Gly-Lys-Pro-Ile-Leu-PhePhe-Arg-Leu-Lys-(Dnp)-D-Arg-NH2 as substrate pretreated for 15 mins followed by substrate addition measured at 5 mins interval for 120 mins by fluorescence assay | Homo sapiens | 9 | ALA4138143 | assay format | Scientific Literature | |
| 3. | ALA4141001 | B | Inhibition of recombinant human C-terminal His10-tagged cathepsin E (Gln18 to Pro396 residues) using Mca-Gly-Lys-Pro-Ile-Leu-PhePhe-Arg-Leu-Lys-(Dnp)-D-Arg-NH2 as substrate pretreated for 15 mins followed by substrate addition measured at 5 mins interval for 120 mins by fluorescence assay | Homo sapiens | 9 | ALA4138143 | single protein format | Scientific Literature | |
| 4. | ALA4141002 | B | Selectivity ratio of IC50 for human liver cathepsin D to IC50 for recombinant human C-terminal His10-tagged cathepsin E (Gln18 to Pro396 residues) | Homo sapiens | 9 | ALA4138143 | assay format | Scientific Literature | |
| 5. | ALA4141003 | B | Inhibition of cathepsin D in human MDA-MB-231 cell lysates using Mca-Gly-Lys-Pro-Ile-Leu-PhePhe-Arg-Leu-Lys-(Dnp)-D-Arg-NH2 as substrate pretreated for 15 mins followed by substrate addition measured at 30 secs interval for 30 mins by fluorescence assay | Homo sapiens | 2 | ALA4138143 | single protein format | Scientific Literature | |
| 6. | ALA4141004 | B | Selectivity ratio of IC50 for cathepsin E to IC50 for cathepsin D in human MDA-MB-231 cell lysates | Homo sapiens | 2 | ALA4138143 | assay format | Scientific Literature | |
| 7. | ALA4141005 | B | Inhibition of cathepsin D in human MDA-MB-231 cells using Mca-Gly-Lys-Pro-Ile-Leu-PhePhe-Arg-Leu-Lys-(Dnp)-D-Arg-NH2 as substrate pretreated for 4 hrs followed by compound washout and subsequent substrate addition measured at 30 secs interval for 30 mins by fluorescence assay | Homo sapiens | 3 | ALA4138143 | cell-based format | Scientific Literature | |
| 8. | ALA4141006 | B | Inhibition of cathepsin E in human MDA-MB-231 cells using Mca-Gly-Lys-Pro-Ile-Leu-PhePhe-Arg-Leu-Lys-(Dnp)-D-Arg-NH2 as substrate pretreated for 4 hrs followed by compound washout and subsequent substrate addition measured at 30 secs interval for 30 mins by fluorescence assay | Homo sapiens | 3 | ALA4138143 | cell-based format | Scientific Literature | |
| 9. | ALA4141007 | B | Inhibition of cathepsin D in human MDA-MB-231 cells using Mca-Gly-Lys-Pro-Ile-Leu-PhePhe-Arg-Leu-Lys-(Dnp)-D-Arg-NH2 as substrate pretreated for 4 hrs followed by compound washout and subsequent 10 mins trypsinization prior to substrate addition measured at 30 secs interval for 30 mins by fluorescence assay | Homo sapiens | 2 | ALA4138143 | cell-based format | Scientific Literature | |
| 10. | ALA4141008 | B | Inhibition of cathepsin E in human MDA-MB-231 cells using Mca-Gly-Lys-Pro-Ile-Leu-PhePhe-Arg-Leu-Lys-(Dnp)-D-Arg-NH2 as substrate pretreated for 4 hrs followed by compound washout and subsequent 10 mins trypsinization prior to substrate addition measured at 30 secs interval for 30 mins by fluorescence assay | Homo sapiens | 2 | ALA4138143 | cell-based format | Scientific Literature | |
| 11. | ALA4141009 | B | Inhibition of secreted cathepsin E in human MDA-MB-231 cells using Mca-Gly-Lys-Pro-Ile-Leu-PhePhe-Arg-Leu-Lys-(Dnp)-D-Arg-NH2 as substrate pretreated for 3 days under pH 6.6 conditioned media followed by substrate addition measured at 5 mins interval for 2 hrs by fluorescence assay | Homo sapiens | 3 | ALA4138143 | cell-based format | Scientific Literature | |
| 12. | ALA4141010 | B | Inhibition of human liver cathepsin D at 5 uM using recombinant human cystatin-C as substrate measured after 3 hrs by silver staining based SDS-PAGE analysis | Homo sapiens | 3 | ALA4138143 | assay format | Scientific Literature | |
| 13. | ALA4141011 | B | Inhibition of human liver cathepsin D at 1 uM using recombinant human cystatin-C as substrate measured after 3 hrs by silver staining based SDS-PAGE analysis | Homo sapiens | 3 | ALA4138143 | assay format | Scientific Literature | |
| 14. | ALA4141012 | B | Inhibition of human liver cathepsin D at 1 uM using recombinant PAI-1 as substrate measured after 3 hrs by silver staining based SDS-PAGE analysis | Homo sapiens | 3 | ALA4138143 | assay format | Scientific Literature | |
| 15. | ALA4141013 | B | Inhibition of cathepsin D in human MDA-MB-231 cells assessed as increase in secreted cystatin-C level at 100 uM after 3 days under pH 6.6 conditioned media by ELISA relative to control | Homo sapiens | 2 | ALA4138143 | cell-based format | Scientific Literature | |
| 16. | ALA4141015 | B | Inhibition of cathepsin D in human MDA-MB-231 cells assessed as reduction in cysteine cathepsin activity at 10 uM using Z-Phe-Arg-AMC as substrate pretreated for 3 days under pH 6.6 conditioned media followed by substrate addition measured at 5 mins interval for 2 hrs by ELISA relative to control | Homo sapiens | 1 | ALA4138143 | cell-based format | Scientific Literature | |
| 17. | ALA4141017 | A | Cytotoxicity against human MDA-MB-231 cells assessed as effect on cell viability up to 10 uM after 72 hrs under pH 6.6 conditioned media by MTT assay | Homo sapiens | 3 | ALA4138143 | cell-based format | Scientific Literature | |
| 18. | ALA4141018 | B | Inhibition of cathepsin D in human MDA-MB-231 cells assessed as increase in secreted PAI-1 level at 100 uM after 3 days under pH 6.6 conditioned media by ELISA | Homo sapiens | 2 | ALA4138143 | cell-based format | Scientific Literature | |
| 19. | ALA4141022 | B | Inhibition of cathepsin D in human MDA-MB-231 cells assessed as reduction in tPA residual activity up to 10 uM incubated for 3 days under pH 6.6 conditioned media measured at 1 hr interval for 8 hrs by chromogenic method relative to control | Homo sapiens | 2 | ALA4138143 | cell-based format | Scientific Literature | |
| 20. | ALA4141023 | F | Antimigratory activity against human MDA-MB-231 cells at 5 uM after 48 hrs by crystal violet staining based microscopic method relative to control | Homo sapiens | 3 | ALA4138143 | cell-based format | Scientific Literature |
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