Agonist activity at human GPR109a expressed in HEK293 cells harboring glosensor-22F cAMP plasmid DNA assessed as inhibition of forskolin-stimulated cAMP accumulation preincubated with cells followed by forskolin addition measured after 10 mins by luminescence assay
Anti-obesity activity in high fat diet-induced obese C57BL/6J mouse model assessed as fat mass at 20 mg/kg, po for 6 weeks by NMR-MRI analysis (Rvb = 16.52 +/- 1.154 g)
Anti-obesity activity in high fat diet-induced obese C57BL/6J mouse model assessed as lean mass at 20 mg/kg, po for 6 weeks by NMR-MRI analysis (Rvb = 25.58 +/- 0.6789 g)
Anti-obesity activity in high fat diet-induced obese C57BL/6J mouse model assessed as fasting glucose level at 20 mg/kg, po for 6 weeks (Rvb = 93.17 +/- 5.793 mg/dL)
Anti-obesity activity in high fat diet-induced obese C57BL/6J mouse model assessed as plasma leptin level at 20 mg/kg, po for 6 weeks (Rvb = 1751 +/- 325.4 pg/mg)
Antiobesity activity in high fat diet-induced obese C57BL/6J mouse model assessed as improvement in glucose tolerance at 20 mg/kg, po measured post glucose challenge by intraperitoneal glucose tolerance test