| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4141606 | B | Binding affinity to human Keap1 Kelch domain (322 to 609 residues) expressed in Escherichia coli (DE3) by isothermal titration calorimetry | Homo sapiens | 2 | assay format | Scientific Literature | ||
| 2. | ALA4141607 | B | Binding affinity to human biotin-labeled Keap1 Kelch domain (322 to 609 residues) expressed in Escherichia coli (DE3) by biolayer interferometry | Homo sapiens | 2 | assay format | Scientific Literature | ||
| 3. | ALA4141608 | B | Binding affinity to human biotin-labeled Keap1 Kelch domain (322 to 609 residues) expressed in Escherichia coli (DE3) assessed as association rate constant by biolayer interferometry | Homo sapiens | 2 | assay format | Scientific Literature | ||
| 4. | ALA4141609 | B | Binding affinity to human biotin-labeled Keap1 Kelch domain (322 to 609 residues) expressed in Escherichia coli (DE3) assessed as dissociation rate constant by biolayer interferometry | Homo sapiens | 2 | assay format | Scientific Literature | ||
| 5. | ALA4141610 | B | Inhibition of FITC-labeled Nrf2 binding to human Keap1 Kelch domain (322 to 609 residues) expressed in Escherichia coli (DE3) after 30 mins by fluorescence polarization assay | Homo sapiens | 2 | assay format | Scientific Literature | ||
| 6. | ALA4141615 | B | Inhibition of Keap1/Nrf2 protein-protein interaction in mouse RAW264.7 cells assessed as increase in NQO1 levels up to 10 uM after 8 hrs by Western blot analysis | Mus musculus | 2 | cell-based format | Scientific Literature | ||
| 7. | ALA4141616 | B | Inhibition of Keap1/Nrf2 protein-protein interaction in mouse RAW264.7 cells assessed as increase in HO-1 levels up to 10 uM after 8 hrs by Western blot analysis | Mus musculus | 2 | cell-based format | Scientific Literature | ||
| 8. | ALA4141617 | B | Inhibition of Keap1/Nrf2 protein-protein interaction in mouse RAW264.7 cells assessed as increase in GCLM levels up to 10 uM after 8 hrs by Western blot analysis | Mus musculus | 2 | cell-based format | Scientific Literature | ||
| 9. | ALA4141618 | B | Inhibition of Keap1/Nrf2 protein-protein interaction in mouse RAW264.7 cells assessed as restoration of LPS-induced reduction of GSH/GSSG ratio up to 10 uM preincubated for 10 hrs followed by LPS stimulation for 12 hrs | Mus musculus | 2 | cell-based format | Scientific Literature | ||
| 10. | ALA4141619 | F | Upregulation of SOD protein expression in mouse RAW264.7 cells at 1 uM after 8 hrs by Western blot analysis | Mus musculus | 1 | cell-based format | Scientific Literature | ||
| 11. | ALA4141620 | F | Upregulation of GSH-Px protein expression in mouse RAW264.7 cells at 1 uM after 8 hrs by Western blot analysis | Mus musculus | 1 | cell-based format | Scientific Literature | ||
| 12. | ALA4141621 | F | Inhibition of LPS-induced increase in ROS levels in mouse RAW264.7 cells up to 10 uM preincubated for 10 hrs followed by LPS stimulation for 12 hrs by cH2DCF-DA dye-based fluorescent microscopy | Mus musculus | 1 | cell-based format | Scientific Literature | ||
| 13. | ALA4141622 | F | Anti-inflammatory activity in mouse RAW264.7 cells assessed as restoration of LPS-induced decrease in SOD activity at 10 uM | Mus musculus | 1 | cell-based format | Scientific Literature | ||
| 14. | ALA4141623 | F | Anti-inflammatory activity in mouse RAW264.7 cells assessed restoration of LPS-induced decrease in GSH-Px activity at 10 uM | Mus musculus | 1 | cell-based format | Scientific Literature | ||
| 15. | ALA4141624 | F | Anti-inflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced IL-1beta production up to 10 uM pretreated for 10 hrs followed by LPS stimulation for 12 hrs by ELISA | Mus musculus | 1 | cell-based format | Scientific Literature | ||
| 16. | ALA4141625 | F | Anti-inflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced IL-18 production up to 10 uM pretreated for 10 hrs followed by LPS stimulation for 12 hrs by ELISA | Mus musculus | 1 | cell-based format | Scientific Literature | ||
| 17. | ALA4141626 | F | Anti-inflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced TNFalpha production up to 10 uM pretreated for 10 hrs followed by LPS stimulation for 12 hrs by ELISA | Mus musculus | 1 | cell-based format | Scientific Literature | ||
| 18. | ALA4141627 | F | Anti-inflammatory activity in mouse RAW264.7 cells assessed as reduction in LPS-induced NO production by measuring NO level at 10 uM preincubated for 10 hrs followed by LPS stimulation for 12 hrs by Griess assay (Rvb = 50 uM) | Mus musculus | 1 | cell-based format | Scientific Literature | ||
| 19. | ALA4141628 | F | Anti-inflammatory activity in mouse RAW264.7 cells assessed inhibition of LPS-induced increase in MPO activity at 10 uM | Mus musculus | 1 | cell-based format | Scientific Literature | ||
| 20. | ALA4141629 | B | Inhibition of Keap1 interaction with Nrf2 (unknown origin) in human HepG2 cells harboring Nrf2/ARE-Luc assessed as Nrf2 activation after 12 hrs by luciferase reporter gene assay | Homo sapiens | 1 | cell-based format | Scientific Literature |
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