# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4150505 | B | Binding affinity to mouse N-terminal Halo-fused/biotin-labelled Lin28A expressed in Escherichia coli BL21(DE3) assessed as inhibition of protein interaction with pre-Let-7d microRNA measured after 1 hr by cat-ELCCA | Mus musculus | 26 | ALA4145627 | assay format | Scientific Literature | |
2. | ALA4150506 | B | Binding affinity to mouse N-terminal Halo-fused/biotin-labelled Lin28A expressed in Escherichia coli BL21(DE3) assessed as inhibition of protein interaction with pre-Let-7d microRNA by electrophoretic mobility shift assay | Mus musculus | 2 | ALA4145627 | assay format | Scientific Literature | |
3. | ALA4150507 | B | Binding affinity to human N-terminal Halo-fused/biotin-labelled Lin28B expressed in Escherichia coli BL21(DE3) assessed as inhibition of protein interaction with pre-Let-7d microRNA measured after 1 hr by cat-ELCCA | Homo sapiens | 2 | ALA4145627 | assay format | Scientific Literature | |
4. | ALA4150508 | B | Binding affinity to mouse N-terminal Halo-fused/biotin-labelled Lin28A expressed in Escherichia coli BL21(DE3) assessed as inhibition of protein interaction with pre-Let-7a microRNA at 20 to 50 uM by electrophoretic mobility shift assay | Mus musculus | 2 | ALA4145627 | assay format | Scientific Literature | |
5. | ALA4150509 | B | Binding affinity to mouse N-terminal Halo-fused/biotin-labelled Lin28A expressed in Escherichia coli BL21(DE3) assessed as inhibition of protein interaction with pre-Let-7b microRNA at 20 to 50 uM by electrophoretic mobility shift assay | Mus musculus | 2 | ALA4145627 | assay format | Scientific Literature | |
6. | ALA4150510 | B | Binding affinity to mouse N-terminal Halo-fused/biotin-labelled Lin28A expressed in Escherichia coli BL21(DE3) at 18.7 to 75 uM by surface plasmon resonance analysis | Mus musculus | 1 | ALA4145627 | assay format | Scientific Literature | |
7. | ALA4150511 | B | Binding affinity to synthetic biotinylated pre-let-7d microRNA at 6.25 to 100 uM by surface plasmon resonance analysis | 1 | ALA4145627 | assay format | Scientific Literature | ||
8. | ALA4150512 | B | Binding affinity to dicer (unknown origin) assessed as inhibition of dicer-meditated pre-let-7d microRNA maturation at 100 uM by electrophoretic mobility shift assay | Homo sapiens | 2 | ALA4145627 | single protein format | Scientific Literature | |
9. | ALA4150513 | B | Inhibition of eIF4E-4E-BP1 (unknown origin) interaction at 100 uM by cat-ELCCA | Homo sapiens | 1 | ALA4145627 | protein format | Scientific Literature |