| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4156262 | B | Inhibition of recombinant His-tagged SIRT2 (56 to 356 residues) (unknown origin) expressed in Escherichia coli Transetta(DE3) using AcIQF as substrate preincubated for 30 mins followed by substrate and NAD+ addition measured after 2 hrs by fluorescence assay | Homo sapiens | 40 | ALA4152324 | assay format | Scientific Literature | |
| 2. | ALA4156263 | B | Inhibition of recombinant human N-terminal His6-tagged SIRT1 (241 to 512 residues) expressed in Escherichia coli Transetta(DE3) using AcIQF as substrate preincubated for 30 mins followed by substrate and NAD+ addition measured after 2 hrs by fluorescence assay | Homo sapiens | 1 | ALA4152324 | assay format | Scientific Literature | |
| 3. | ALA4156264 | B | Inhibition of recombinant human N-terminal His6-tagged SIRT3 (122 to 391 residues) expressed in Escherichia coli Transetta(DE3) using AcIQF as substrate preincubated for 30 mins followed by substrate and NAD+ addition measured after 2 hrs by fluorescence assay | Homo sapiens | 1 | ALA4152324 | assay format | Scientific Literature | |
| 4. | ALA4156265 | B | Inhibition of recombinant human N-terminal His6-tagged SIRT5 (34 to 356 residues) expressed in Escherichia coli Transetta(DE3) using GluIQF as substrate preincubated for 30 mins followed by substrate and NAD+ addition measured after 2 hrs by fluorescence assay | Homo sapiens | 1 | ALA4152324 | assay format | Scientific Literature | |
| 5. | ALA4156266 | B | Inhibition of recombinant human N-terminal His6-tagged SIRT6 (4 to 296 residues) expressed in Escherichia coli Transetta(DE3) using AcIQF as substrate preincubated for 30 mins followed by substrate and NAD+ addition measured after 2 hrs by fluorescence assay | Homo sapiens | 1 | ALA4152324 | assay format | Scientific Literature | |
| 6. | ALA4156267 | B | Inhibition of recombinant human N-terminal His6-tagged SIRT7 (68 to 354 residues) expressed in Escherichia coli Transetta(DE3) using AcIQF as substrate preincubated for 30 mins followed by substrate and NAD+ addition measured after 2 hrs by fluorescence assay | Homo sapiens | 1 | ALA4152324 | assay format | Scientific Literature | |
| 7. | ALA4156268 | B | Inhibition of SIRT2 in human NCI-H441 cells assessed as increase in acetylated alpha-tubulin level after 72 hrs by Western blot analysis | Homo sapiens | 1 | ALA4152324 | cell-based format | Scientific Literature | |
| 8. | ALA4156270 | F | Antiproliferative activity against human NCI-H441 cells after 72 hrs by MTT assay | Homo sapiens | 2 | ALA4152324 | cell-based format | Scientific Literature | |
| 9. | ALA4156271 | F | Anticlonogenic activity against human NCI-H441 cells at 3 to 30 uM after 2 weeks by crystal violet staining based assay | Homo sapiens | 1 | ALA4152324 | cell-based format | Scientific Literature | |
| 10. | ALA4156273 | F | Antiproliferative activity against human NCI-H441 cells assessed as reduction in EdU incorporation at 30 uM after 48 hrs by DAPI staining based fluorescence microscopic method | Homo sapiens | 2 | ALA4152324 | cell-based format | Scientific Literature | |
| 11. | ALA4156274 | F | Induction of apoptosis in human NCI-H441 cells assessed as live cells at 30 uM after 48 hrs by Annexin V-FITC/propidium iodide staining based flow cytometry (Rvb = 88.5%) | Homo sapiens | 2 | ALA4152324 | cell-based format | Scientific Literature | |
| 12. | ALA4156275 | F | Induction of apoptosis in human NCI-H441 cells assessed as early apoptotic cells at 30 uM after 48 hrs by Annexin V-FITC/propidium iodide staining based flow cytometry (Rvb = 2.47%) | Homo sapiens | 2 | ALA4152324 | cell-based format | Scientific Literature | |
| 13. | ALA4156276 | F | Induction of apoptosis in human NCI-H441 cells assessed as late apoptotic cells at 30 uM after 48 hrs by Annexin V-FITC/propidium iodide staining based flow cytometry (Rvb = 7.25%) | Homo sapiens | 2 | ALA4152324 | cell-based format | Scientific Literature | |
| 14. | ALA4156277 | F | Induction of apoptosis in human NCI-H441 cells assessed as necrotic cells at 30 uM after 48 hrs by Annexin V-FITC/propidium iodide staining based flow cytometry (Rvb = 1.79%) | Homo sapiens | 2 | ALA4152324 | cell-based format | Scientific Literature | |
| 15. | ALA4156278 | F | Antimigratory activity against human NCI-H441 cells assessed as reduction in wound closure at 10 to 30 uM after 24 hrs by light microscopic method | Homo sapiens | 1 | ALA4152324 | cell-based format | Scientific Literature | |
| 16. | ALA4156280 | F | Anti-invasive activity against human NCI-H441 cells after 48 hrs by crystal violet staining based transwell assay | Homo sapiens | 1 | ALA4152324 | cell-based format | Scientific Literature |
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