| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4131163 | B | Substrate activity at NQO1 in human A549 cells assessed as induction of ROS-induced p38 phosphorylation at T180/Y182 after 3 hrs by Western blot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 2. | ALA4131164 | B | Substrate activity at recombinant human NQO1 expressed in Escherichia coli assessed as reduction rate by measuring umol of NADPH utilized per umol of enzyme at 10 uM preincubated for 3 mins followed by NADPH addition measured at 2 secs interval for 5 mins by UV-Vis spectroscopy | Homo sapiens | 23 | assay format | Scientific Literature | ||
| 3. | ALA4131165 | A | Substrate activity at human CPR expressed in baculovirus expression system assessed as reduction rate by measuring umol of NADPH utilized per umol of enzyme at 10 uM preincubated for 3 mins followed by NADPH addition measured at 2 secs interval for 5 mins by UV-Vis spectroscopy | Homo sapiens | 8 | assay format | Scientific Literature | ||
| 4. | ALA4131166 | B | Selectivity ratio for substrate activity at recombinant human NQO1 to substrate activity at human CPR | Homo sapiens | 8 | assay format | Scientific Literature | ||
| 5. | ALA4131167 | P | Lipophilicity, log D of the compound at pH 7.4 | 8 | small-molecule physicochemical format | Scientific Literature | |||
| 6. | ALA4131168 | P | Intrinsic solubility of the compound | 8 | small-molecule physicochemical format | Scientific Literature | |||
| 7. | ALA4131169 | P | Solubility of the compound in diluted HCl buffer at pH 3 | 8 | small-molecule physicochemical format | Scientific Literature | |||
| 8. | ALA4131170 | A | Effective permeability of the compound at pH 7.4 by PAMPA assay | 8 | assay format | Scientific Literature | |||
| 9. | ALA4131171 | B | Substrate activity at recombinant human NQO1 expressed in Escherichia coli assessed as Kcat to Km ratio at 10 uM preincubated for 3 mins followed by NADPH addition measured at 2 secs interval for 5 mins by UV-Vis spectroscopy | Homo sapiens | 8 | assay format | Scientific Literature | ||
| 10. | ALA4131172 | A | Substrate activity at human Trx expressed in Escherichia coli at 10 uM preincubated for 3 mins followed by NADPH addition measured at 2 secs interval for 5 mins by UV-Vis spectroscopy | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 11. | ALA4131173 | A | Substrate activity at CBR1 (unknown origin) at 10 uM preincubated for 3 mins followed by NADPH addition measured at 2 secs interval for 5 mins by UV-Vis spectroscopy | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 12. | ALA4131174 | F | Cytotoxicity against human A549 cells assessed as cell growth inhibition preincubated for 24 hrs followed by compound washout measured after 72 hrs by MTT assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 13. | ALA4131175 | F | Cytotoxicity against human NCI-H1299 cells assessed as assessed as cell growth inhibition for 24 hrs followed by compound washout measured after 72 hrs by MTT assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 14. | ALA4131176 | F | Cytotoxicity against human MCF7 cells assessed as cell growth inhibition preincubated for 24 hrs followed by compound washout measured after 72 hrs by MTT assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 15. | ALA4131177 | F | Cytotoxicity against human HepG2 cells assessed as cell growth inhibition preincubated for 24 hrs followed by compound washout measured after 72 hrs by MTT assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 16. | ALA4131178 | F | Cytotoxicity against human HCT116 cells assessed as cell growth inhibition preincubated for 24 hrs followed by compound washout measured after 72 hrs by MTT assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 17. | ALA4131179 | F | Cytotoxicity against HEK293 cells assessed as cell growth inhibition preincubated for 24 hrs followed by compound washout measured after 72 hrs by MTT assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 18. | ALA4131180 | F | Cytotoxicity against human A549/TR cells assessed as cell growth inhibition preincubated for 24 hrs followed by compound washout measured after 72 hrs by MTT assay | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
| 19. | ALA4131181 | F | Cytotoxicity against human A549 cells assessed as cell growth inhibition preincubated for 24 hrs followed by compound washout measured after 72 hrs in presence of NQO1 inhibitor DIC by MTT assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 20. | ALA4131182 | F | Cytotoxicity against human NCI-H596 cells assessed as cell growth inhibition preincubated for 24 hrs followed by compound washout measured after 72 hrs by MTT assay | Homo sapiens | 1 | cell-based format | Scientific Literature |
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