#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4154343	Binding	Inhibition of calf thymus TOP1-mediated relaxation of supercoiled pBR322 DNA at 25 uM after 30 mins by agarose gel electrophoresis relative to camptothecin	Bos taurus	24	ALA4152240	single protein format	Scientific Literature
2.	ALA4154350	Binding	Induction of unwinding effect of Escherichia coli relaxed pBR322 DNA at 1 to 9 uM preincubated with DNA for 10 mins followed by excess calf thymus TOP1 addition and measured after 30 mins by agarose gel electrophoresis	Escherichia coli	25	ALA4152240	nucleic acid format	Scientific Literature
3.	ALA4154351	Binding	Induction of unwinding effect of Escherichia coli supercoiled pBR322 DNA at 1 to 9 uM preincubated with DNA for 10 mins followed by excess calf thymus TOP1 addition and measured after 30 mins by agarose gel electrophoresis	Escherichia coli	25	ALA4152240	nucleic acid format	Scientific Literature
4.	ALA4154352	Functional	Cytotoxicity against human HCT116 cells assessed as growth inhibition after 72 hrs by MTT assay	Homo sapiens	25	ALA4152240	cell-based format	Scientific Literature
5.	ALA4154353	Functional	Cytotoxicity against human CCRF-CEM cells assessed as growth inhibition after 72 hrs by MTT assay	Homo sapiens	25	ALA4152240	cell-based format	Scientific Literature
6.	ALA4154354	Functional	Cytotoxicity against human A549 cells assessed as growth inhibition after 72 hrs by MTT assay	Homo sapiens	25	ALA4152240	cell-based format	Scientific Literature
7.	ALA4154355	Functional	Cytotoxicity against human HuH7 cells assessed as growth inhibition after 72 hrs by MTT assay	Homo sapiens	25	ALA4152240	cell-based format	Scientific Literature
8.	ALA4154356	Functional	Cytotoxicity against human DU145 cells assessed as growth inhibition after 72 hrs by MTT assay	Homo sapiens	25	ALA4152240	cell-based format	Scientific Literature