#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4155117	B	Inhibition of Escherichia coli C-terminal His6-tagged DNA gyrase B expressed in Escherichia coli BL21(DE3) preincubated for 10 mins followed by ATP addition measured after 30 mins by purine nucleoside phosphorylase enzyme coupled spectrophotometric method	Escherichia coli	16	ALA4152269	assay format	Scientific Literature
2.	ALA4155118	B	Inhibition of Escherichia coli C-terminal His6-tagged topoisomerase-4 expressed in Escherichia coli BL21(DE3) preincubated for 10 mins followed by ATP addition measured after 30 mins by purine nucleoside phosphorylase enzyme coupled spectrophotometric method	Escherichia coli	7	ALA4152269	assay format	Scientific Literature
3.	ALA4155124	B	Inhibition of Escherichia coli C-terminal His6-tagged DNA gyrase B expressed in Escherichia coli BL21(DE3) assessed as residual activity at 1 uM preincubated for 10 mins followed by ATP addition measured after 30 mins by purine nucleoside phosphorylase enzyme coupled spectrophotometric method relative to control	Escherichia coli	39	ALA4152269	assay format	Scientific Literature
4.	ALA4155126	B	Inhibition of Staphylococcus aureus DNA gyrase B preincubated for 10 mins followed by ATP addition measured after 30 mins by purine nucleoside phosphorylase enzyme coupled spectrophotometric method	Staphylococcus aureus	11	ALA4152269	single protein format	Scientific Literature
5.	ALA4155130	B	Inhibition of Staphylococcus aureus topoisomerase-4 assessed as residual activity preincubated for 10 mins followed by ATP addition measured after 30 mins by purine nucleoside phosphorylase enzyme coupled spectrophotometric method	Staphylococcus aureus	9	ALA4152269	assay format	Scientific Literature
6.	ALA4155131	F	Growth inhibition of Staphylococcus aureus ATCC 25923 at 50 uM after 24 hrs by broth microdilution method relative to control	Staphylococcus aureus	28	ALA4152269	organism-based format	Scientific Literature
7.	ALA4155132	F	Growth inhibition of Enterococcus faecalis ATCC 29212 at 50 uM after 24 hrs by broth microdilution method relative to control	Enterococcus faecalis	28	ALA4152269	organism-based format	Scientific Literature
8.	ALA4155133	F	Growth inhibition of Escherichia coli ATCC 25922 at 50 uM after 24 hrs by broth microdilution method relative to control	Escherichia coli	28	ALA4152269	organism-based format	Scientific Literature
9.	ALA4155134	F	Growth inhibition of Pseudomonas aeruginosa ATCC 27853 at 50 uM after 24 hrs by broth microdilution method relative to control	Pseudomonas aeruginosa	28	ALA4152269	organism-based format	Scientific Literature
10.	ALA4155135	F	Growth inhibition of tolC deficient Escherichia coli JW5503 at 50 uM after 24 hrs by broth microdilution method relative to control	Escherichia coli	28	ALA4152269	organism-based format	Scientific Literature
11.	ALA4155136	F	Growth inhibition of lpxC deficient Escherichia coli JD17464 at 50 uM after 24 hrs by broth microdilution method relative to control	Escherichia coli	28	ALA4152269	organism-based format	Scientific Literature
12.	ALA4155137	F	Antibacterial activity against Staphylococcus aureus ATCC 25923 after 18 hrs by broth microdilution method	Staphylococcus aureus	12	ALA4152269	organism-based format	Scientific Literature
13.	ALA4155138	F	Antibacterial activity against Enterococcus faecalis ATCC 29212 after 18 hrs by broth microdilution method	Enterococcus faecalis	12	ALA4152269	organism-based format	Scientific Literature
14.	ALA4155139	F	Antibacterial activity against tolC deficient Escherichia coli JW5503 after 18 hrs by broth microdilution method	Escherichia coli	12	ALA4152269	organism-based format	Scientific Literature