| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4154343 | Binding | Inhibition of calf thymus TOP1-mediated relaxation of supercoiled pBR322 DNA at 25 uM after 30 mins by agarose gel electrophoresis relative to camptothecin | Bos taurus | 24 | ALA4152240 | single protein format | Scientific Literature | |
| 2. | ALA4154344 | Binding | Inhibition of calf thymus TOP1-mediated relaxation of supercoiled pBR322 DNA after 30 mins by agarose gel electrophoresis | Bos taurus | 3 | ALA4152240 | single protein format | Scientific Literature | |
| 3. | ALA4154345 | Binding | Inhibition of calf thymus TOP1-mediated relaxation of supercoiled pBR322 DNA at 125 uM after 30 mins by agarose gel electrophoresis relative to control | Bos taurus | 4 | ALA4152240 | single protein format | Scientific Literature | |
| 4. | ALA4154347 | Binding | Poison activity at calf thymus TOP1 assessed as trapping of transient enzyme-DNA covalent complex by measuring ratio of nicked DNA at 25 uM using supercoiled pBR322 DNA after 30 mins by agarose gel electrophoresis (Rvb = 47%) | Bos taurus | 4 | ALA4152240 | single protein format | Scientific Literature | |
| 5. | ALA4154348 | Binding | Poison activity at calf thymus TOP1 assessed as trapping of transient enzyme-DNA covalent complex by measuring ratio of nicked DNA at 50 uM using supercoiled pBR322 DNA after 30 mins by agarose gel electrophoresis (Rvb = 47%) | Bos taurus | 4 | ALA4152240 | single protein format | Scientific Literature | |
| 6. | ALA4154350 | Binding | Induction of unwinding effect of Escherichia coli relaxed pBR322 DNA at 1 to 9 uM preincubated with DNA for 10 mins followed by excess calf thymus TOP1 addition and measured after 30 mins by agarose gel electrophoresis | Escherichia coli | 25 | ALA4152240 | nucleic acid format | Scientific Literature | |
| 7. | ALA4154351 | Binding | Induction of unwinding effect of Escherichia coli supercoiled pBR322 DNA at 1 to 9 uM preincubated with DNA for 10 mins followed by excess calf thymus TOP1 addition and measured after 30 mins by agarose gel electrophoresis | Escherichia coli | 25 | ALA4152240 | nucleic acid format | Scientific Literature | |
| 8. | ALA4154352 | Functional | Cytotoxicity against human HCT116 cells assessed as growth inhibition after 72 hrs by MTT assay | Homo sapiens | 25 | ALA4152240 | cell-based format | Scientific Literature | |
| 9. | ALA4154353 | Functional | Cytotoxicity against human CCRF-CEM cells assessed as growth inhibition after 72 hrs by MTT assay | Homo sapiens | 25 | ALA4152240 | cell-based format | Scientific Literature | |
| 10. | ALA4154354 | Functional | Cytotoxicity against human A549 cells assessed as growth inhibition after 72 hrs by MTT assay | Homo sapiens | 25 | ALA4152240 | cell-based format | Scientific Literature | |
| 11. | ALA4154355 | Functional | Cytotoxicity against human HuH7 cells assessed as growth inhibition after 72 hrs by MTT assay | Homo sapiens | 25 | ALA4152240 | cell-based format | Scientific Literature | |
| 12. | ALA4154356 | Functional | Cytotoxicity against human DU145 cells assessed as growth inhibition after 72 hrs by MTT assay | Homo sapiens | 25 | ALA4152240 | cell-based format | Scientific Literature | |
| 13. | ALA4154367 | Functional | Induction of apoptosis in human HCT116 cells assessed as early apoptotic cells at 1 uM after 12 hrs by annexin V-FITC/propidium iodide staining-based flow cytometric method (Rvb = 0.47 to 1.19%) | Homo sapiens | 3 | ALA4152240 | cell-based format | Scientific Literature | |
| 14. | ALA4154368 | Functional | Induction of apoptosis in human HCT116 cells assessed as late apoptotic cells at 1 uM after 12 hrs by annexin V-FITC/propidium iodide staining-based flow cytometric method (Rvb = 0.34 to 0.81%) | Homo sapiens | 3 | ALA4152240 | cell-based format | Scientific Literature | |
| 15. | ALA4154369 | Functional | Induction of apoptosis in human HCT116 cells assessed as viable cells at 1 uM after 12 hrs by annexin V-FITC/propidium iodide staining-based flow cytometric method (Rvb = 97.60 to 98.95%) | Homo sapiens | 3 | ALA4152240 | cell-based format | Scientific Literature | |
| 16. | ALA4154370 | Functional | Induction of apoptosis in human HCT116 cells assessed as early apoptotic cells at 3 uM after 12 hrs by annexin V-FITC/propidium iodide staining-based flow cytometric method (Rvb = 0.47 to 1.19%) | Homo sapiens | 3 | ALA4152240 | cell-based format | Scientific Literature | |
| 17. | ALA4154371 | Functional | Induction of apoptosis in human HCT116 cells assessed as late apoptotic cells at 3 uM after 12 hrs by annexin V-FITC/propidium iodide staining-based flow cytometric method (Rvb = 0.34 to 0.81%) | Homo sapiens | 3 | ALA4152240 | cell-based format | Scientific Literature | |
| 18. | ALA4154372 | Functional | Induction of apoptosis in human HCT116 cells assessed as viable cells at 3 uM after 12 hrs by annexin V-FITC/propidium iodide staining-based flow cytometric method (Rvb = 97.60 to 98.95%) | Homo sapiens | 3 | ALA4152240 | cell-based format | Scientific Literature | |
| 19. | ALA4154373 | Functional | Induction of apoptosis in human HCT116 cells assessed as early apoptotic cells at 9 uM after 12 hrs by annexin V-FITC/propidium iodide staining-based flow cytometric method (Rvb = 0.47 to 1.19%) | Homo sapiens | 3 | ALA4152240 | cell-based format | Scientific Literature | |
| 20. | ALA4154374 | Functional | Induction of apoptosis in human HCT116 cells assessed as late apoptotic cells at 9 uM after 12 hrs by annexin V-FITC/propidium iodide staining-based flow cytometric method (Rvb = 0.34 to 0.81%) | Homo sapiens | 3 | ALA4152240 | cell-based format | Scientific Literature |
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