#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4138596	Binding	Inhibition of calf thymus topoisomerase 1 assessed as suppression of relaxation of supercoiled pBR322 DNA at 100 uM incubated for 0.5 hrs by bromophenol blue and xylene cyanol FF staining based electrophoresis method	Bos taurus	14	ALA4138059	single protein format	Scientific Literature
2.	ALA4138597	Binding	Potency index, ratio of enzyme inhibition of securinine to enzyme inhibition of test compound for inhibition of calf thymus topoisomerase 1 assessed as suppression of relaxation of supercoiled pBR322 DNA at 100 uM incubated for 0.5 hrs by bromophenol blue and xylene cyanol FF staining based electrophoresis method	Bos taurus	1	ALA4138059	single protein format	Scientific Literature
3.	ALA4138598	Binding	Inhibition of calf thymus topoisomerase 1 assessed as reduction in topo1/DNA complex formation at 50 to 400 uM pre-incubated for 0.33 hrs before addition of supercoiled pBR322 DNA substrate by EMSA	Bos taurus	1	ALA4138059	single protein format	Scientific Literature
4.	ALA4138600	Binding	Inhibition of calf thymus topoisomerase 1 assessed as increase in nicked DNA at 10 uM using supercoiled pBR322 DNA incubated for 0.5 hrs by ethidium bromide staining based DNA-cleavage assay	Bos taurus	5	ALA4138059	single protein format	Scientific Literature
5.	ALA4138601	Binding	Induction of DNA intercalation assessed as reduction in topoisomerase 1-mediated DNA relaxation at 100 uM using supercoiled pBR322 DNA incubated for 0.5 hrs in presence of calf thymus topoisomerase 1 by electrophoresis method	Escherichia coli	7	ALA4138059	assay format	Scientific Literature