# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4197281 | Functional | Cytotoxicity against human HL60 cells assessed as reduction in cell proliferation after 48 hrs by MTT assay | Homo sapiens | 6 | ALA4196027 | cell-based format | Scientific Literature | |
2. | ALA4197282 | Functional | Cytotoxicity against human K562 cells assessed as reduction in cell proliferation after 48 hrs by MTT assay | Homo sapiens | 6 | ALA4196027 | cell-based format | Scientific Literature | |
3. | ALA4197283 | Functional | Cytotoxicity against human A549 cells assessed as reduction in cell proliferation after 48 hrs by MTT assay | Homo sapiens | 6 | ALA4196027 | cell-based format | Scientific Literature | |
4. | ALA4197284 | Functional | Cytotoxicity against human NCI-H1975 cells assessed as reduction in cell proliferation after 48 hrs by MTT assay | Homo sapiens | 6 | ALA4196027 | cell-based format | Scientific Literature | |
5. | ALA4197285 | Functional | Cytotoxicity against human MGC803 cells assessed as reduction in cell proliferation after 48 hrs by MTT assay | Homo sapiens | 6 | ALA4196027 | cell-based format | Scientific Literature | |
6. | ALA4197286 | ADME | Cytotoxicity against HEK293 cells assessed as reduction in cell proliferation after 48 hrs by MTT assay | Homo sapiens | 6 | ALA4196027 | cell-based format | Scientific Literature | |
7. | ALA4197287 | Functional | Cytotoxicity against human HO8910 cells assessed as reduction in cell proliferation after 48 hrs by MTT assay | Homo sapiens | 6 | ALA4196027 | cell-based format | Scientific Literature | |
8. | ALA4197288 | Functional | Cytotoxicity against human HCT116 cells assessed as reduction in cell proliferation after 48 hrs by MTT assay | Homo sapiens | 6 | ALA4196027 | cell-based format | Scientific Literature | |
9. | ALA4197289 | Binding | Inhibition of DNA topoisomerase-1 (unknown origin) using supercoiled pBR322 DNA as substrate preincubated with enzyme followed by substrate addition measured after 30 mins by ethidium bromide staining based agarose gel electrophoresis method | Homo sapiens | 1 | ALA4196027 | single protein format | Scientific Literature | |
10. | ALA4197290 | Binding | Inhibition of DNA topoisomerase-1 (unknown origin) up to 40 uM using supercoiled pBR322 DNA as substrate preincubated with enzyme followed by substrate addition measured after 30 mins by ethidium bromide staining based agarose gel electrophoresis method | Homo sapiens | 2 | ALA4196027 | single protein format | Scientific Literature | |
11. | ALA4197291 | Binding | Inhibition of DNA topoisomerase-2 (unknown origin) at 100 uM using kinetoplast DNA as substrate measured after 30 mins by ethidium bromide staining based agarose gel electrophoresis method | Homo sapiens | 2 | ALA4196027 | assay format | Scientific Literature | |
12. | ALA4197292 | Binding | Inhibition of calf thymus DNA topoisomerase-1-mediated supercoiled pBR322 DNA relaxation assessed as stabilization of enzyme-DNA complex at 100 uM preincubated with enzyme followed by substrate addition measured after 30 mins by ethidium bromide staining based agarose gel electrophoresis method | Bos taurus | 2 | ALA4196027 | single protein format | Scientific Literature | |
13. | ALA4197294 | Binding | Inhibition of ethidium bromide binding to Escherichia coli pBR322 DNA at 100 uM after 30 mins by fluorescence assay | Escherichia coli | 2 | ALA4196027 | nucleic acid format | Scientific Literature | |
14. | ALA4197295 | Binding | Binding affinity to Escherichia coli pBR322 DNA assessed as red shift by UV-Visible spectrum analysis | Escherichia coli | 1 | ALA4196027 | nucleic acid format | Scientific Literature | |
15. | ALA4197296 | Binding | Binding affinity to Escherichia coli pBR322 DNA assessed as hypochromicity by UV-Visible spectrum analysis | Escherichia coli | 1 | ALA4196027 | nucleic acid format | Scientific Literature | |
16. | ALA4197297 | Binding | Inhibition of DNA topoisomerase-1 (unknown origin) at 10 uM using supercoiled pBR322 DNA as substrate preincubated for 1 to 30 mins followed by substrate addition by ethidium bromide staining based agarose gel electrophoresis method | Homo sapiens | 1 | ALA4196027 | single protein format | Scientific Literature | |
17. | ALA4197298 | Binding | Inhibition of DNA topoisomerase-1 (unknown origin) at 10 uM using supercoiled pBR322 DNA as substrate preincubated for 15 mins followed by substrate addition measured after 30 mins by ethidium bromide staining based agarose gel electrophoresis method | Homo sapiens | 1 | ALA4196027 | single protein format | Scientific Literature | |
18. | ALA4197299 | Functional | Cell cycle arrest in human MGC803 cells assessed as accumulation at G0/G1 phase at 6.25 uM after 24 hrs by propidium iodide staining based flow cytometry (Rvb = 59.68%) | Homo sapiens | 1 | ALA4196027 | cell-based format | Scientific Literature | |
19. | ALA4197300 | Functional | Cell cycle arrest in human MGC803 cells assessed as accumulation at S phase at 6.25 uM after 24 hrs by propidium iodide staining based flow cytometry (Rvb = 31.17%) | Homo sapiens | 1 | ALA4196027 | cell-based format | Scientific Literature | |
20. | ALA4197301 | Functional | Cell cycle arrest in human MGC803 cells assessed as accumulation at G2/M phase at 6.25 uM after 24 hrs by propidium iodide staining based flow cytometry (Rvb = 9.15%) | Homo sapiens | 1 | ALA4196027 | cell-based format | Scientific Literature |